A Negative Feedback Loop That Limits The Ectopic Activation Of A Cell Type-specific Sporulation Sigma Factor Of Bacillus Subtilis

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dc.contributor.author Serrano, M
dc.contributor.author Real, G
dc.contributor.author da Silva Santos, JM
dc.contributor.author Carneiro, J
dc.contributor.author Moran, C
dc.contributor.author Henriques, A
dc.date.accessioned 2012-10-12T03:33:05Z
dc.date.issued 2011-01
dc.identifier.citation Plos Genetics, 2011, 7 (9), pp. 1 - 16
dc.identifier.issn 1553-7390
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/18039
dc.description.abstract Two highly similar RNA polymerase sigma subunits, sigma(F) and sigma(G), govern the early and late phases of forespore-specific gene expression during spore differentiation in Bacillus subtilis. sigma(F) drives synthesis of sigma(G) but the latter only becomes active once engulfment of the forespore by the mother cell is completed, its levels rising quickly due to a positive feedback loop. The mechanisms that prevent premature or ectopic activation of sigma(G) while discriminating between sigma(F) and sigma(G) in the forespore are not fully comprehended. Here, we report that the substitution of an asparagine by a glutamic acid at position 45 of sigma(G) (N45E) strongly reduced binding by a previously characterized anti-sigma factor, CsfB (also known as Gin), in vitro, and increased the activity of sigma(G) in vivo. The N45E mutation caused the appearance of a sub-population of pre-divisional cells with strong activity of sigma(G). CsfB is normally produced in the forespore, under sigma(F) control, but sigGN45E mutant cells also expressed csfB and did so in a sigma(G) -dependent manner, autonomously from sigma(F). Thus, a negative feedback loop involving CsfB counteracts the positive feedback loop resulting from ectopic sigma(G) activity. N45 is invariant in the homologous position of sigma(G) orthologues, whereas its functional equivalent in sigma(F) proteins, E39, is highly conserved. While CsfB does not bind to wildtype sigma(F), a E39N substitution in sigma(F) resulted in efficient binding of CsfB to sigma(F). Moreover, under certain conditions, the E39N alteration strongly restrains the activity of sigma(F) in vivo, in a csfB-dependent manner, and the efficiency of sporulation. Therefore, a single amino residue, N45/E39, is sufficient for the ability of CsfB to discriminate between the two forespore-specific sigma factors in B. subtilis.
dc.format Jessica Robinson
dc.publisher Public Library of Science
dc.relation.isbasedon 10.1371/journal.pgen.1002220
dc.subject TRANSCRIPTION FACTOR SIGMA(G), FORESPORE-SPECIFIC TRANSCRIPTION, BACTERIAL ENDOSPORE FORMATION, GENE-EXPRESSION, RNA-POLYMERASE, FACTOR SPOIIAB, PROMOTER RECOGNITION, MASTER REGULATOR, MOTHER-CELL, PROTEIN, Developmental Biology
dc.subject TRANSCRIPTION FACTOR SIGMA(G); FORESPORE-SPECIFIC TRANSCRIPTION; BACTERIAL ENDOSPORE FORMATION; GENE-EXPRESSION; RNA-POLYMERASE; FACTOR SPOIIAB; PROMOTER RECOGNITION; MASTER REGULATOR; MOTHER-CELL; PROTEIN; Developmental Biology
dc.title A Negative Feedback Loop That Limits The Ectopic Activation Of A Cell Type-specific Sporulation Sigma Factor Of Bacillus Subtilis
dc.type Journal Article
dc.parent Plos Genetics
dc.journal.volume 9
dc.journal.volume 7
dc.journal.number 9 en_US
dc.publocation San Francisco, CA, United States en_US
dc.identifier.startpage 1 en_US
dc.identifier.endpage en_US
dc.identifier.endpage 16 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 030499 Medicinal and Biomolecular Chemistry Not Elsewhere Classified
dc.personcode 0000075561 en_US
dc.personcode 0000075562 en_US
dc.personcode 104615 en_US
dc.personcode 0000075564 en_US
dc.personcode 0000075565 en_US
dc.personcode 0000075566 en_US
dc.percentage 100 en_US
dc.classification.name Medicinal and Biomolecular Chemistry not elsewhere classified en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords TRANSCRIPTION FACTOR SIGMA(G); FORESPORE-SPECIFIC TRANSCRIPTION; BACTERIAL ENDOSPORE FORMATION; GENE-EXPRESSION; RNA-POLYMERASE; FACTOR SPOIIAB; PROMOTER RECOGNITION; MASTER REGULATOR; MOTHER-CELL; PROTEIN en_US
dc.description.keywords TRANSCRIPTION FACTOR SIGMA(G)
dc.description.keywords TRANSCRIPTION FACTOR SIGMA(G)
dc.description.keywords FORESPORE-SPECIFIC TRANSCRIPTION
dc.description.keywords FORESPORE-SPECIFIC TRANSCRIPTION
dc.description.keywords BACTERIAL ENDOSPORE FORMATION
dc.description.keywords BACTERIAL ENDOSPORE FORMATION
dc.description.keywords GENE-EXPRESSION
dc.description.keywords GENE-EXPRESSION
dc.description.keywords RNA-POLYMERASE
dc.description.keywords RNA-POLYMERASE
dc.description.keywords FACTOR SPOIIAB
dc.description.keywords FACTOR SPOIIAB
dc.description.keywords PROMOTER RECOGNITION
dc.description.keywords PROMOTER RECOGNITION
dc.description.keywords MASTER REGULATOR
dc.description.keywords MASTER REGULATOR
dc.description.keywords MOTHER-CELL
dc.description.keywords MOTHER-CELL
dc.description.keywords PROTEIN
dc.description.keywords PROTEIN
dc.description.keywords TRANSCRIPTION FACTOR SIGMA(G)
dc.description.keywords FORESPORE-SPECIFIC TRANSCRIPTION
dc.description.keywords BACTERIAL ENDOSPORE FORMATION
dc.description.keywords GENE-EXPRESSION
dc.description.keywords RNA-POLYMERASE
dc.description.keywords FACTOR SPOIIAB
dc.description.keywords PROMOTER RECOGNITION
dc.description.keywords MASTER REGULATOR
dc.description.keywords MOTHER-CELL
dc.description.keywords PROTEIN
dc.staffid en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science


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