An ecdysone receptor from the pentatomomorphan, Nezara viridula, shows similar affinities for moulting hormones makisterone A and 20-hydroxyecdysone.

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Show simple item record Tohidi-Esfahani, D Graham, LD Hannan, GN Simpson, AM Hill, RJ 2012-10-12T03:33:26Z 2011-02
dc.identifier.citation Insect biochemistry and molecular biology, 2011, 41 (2), pp. 77 - 89
dc.identifier.issn 0965-1748
dc.identifier.other C1 en_US
dc.description.abstract It has been suggested that Pentatomomorpha utilise the C(28) ecdysteroid, makisterone A (MakA), as the major moulting hormone rather than the more common C(27) hormone, 20-hydroxyecdsyone (20E). The present study is the first to examine this postulate at the level of the ecdysone receptor protein, a heterodimer of nuclear receptors EcR and USP. cDNAs encoding two alternatively spliced isoforms of EcR and a single USP were isolated from a high-quality cDNA library prepared from a representative pentatomomorphan, Nezara viridula (Nv). NvEcR and NvUSP were found to group phylogenetically with heteropteran and other insect EcRs and USP/RXRs, respectively. Sequence comparison and phylogenetic analysis of these proteins found them to be distinct from those belonging to other hemipteran ecdysone receptors characterised to date. Co-expression of the His(6)-tagged ligand binding regions (LBRs) of the two NvEcR variants with the FLAG-tagged LBR of NvUSP was achieved in insect cells employing appropriately constructed baculoviruses. The corresponding heterodimers, designated NvE10 and NvE11, were purified by affinity chromatography utilising the His(6) tags on their NvEcR subunits. The heterodimers displayed nanomolar affinity for [(3)H]ponasterone A (K(d) = 6.8-7.5 nM), characteristic of ecdysone receptors. MakA has a similar affinity to 20E for both NvE10 and NvE11, consistent with MakA being a major moulting hormone in N. viridula.
dc.format Print-Electronic
dc.language eng
dc.relation.isbasedon 10.1016/j.ibmb.2010.10.002
dc.title An ecdysone receptor from the pentatomomorphan, Nezara viridula, shows similar affinities for moulting hormones makisterone A and 20-hydroxyecdysone.
dc.type Journal Article
dc.description.version Published
dc.parent Insect biochemistry and molecular biology
dc.journal.volume 2
dc.journal.volume 41
dc.journal.number 2 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 77 en_US
dc.identifier.endpage 89 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 940084
dc.percentage 100 en_US Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity WOS:000287055100001 en_US
dc.description.keywords Animals
dc.description.keywords Hemiptera
dc.description.keywords Ecdysone
dc.description.keywords Ecdysterone
dc.description.keywords Receptors, Cytoplasmic and Nuclear
dc.description.keywords Molting
dc.description.keywords Phylogeny
dc.description.keywords Base Sequence
dc.description.keywords Protein Binding
dc.description.keywords Dimerization
dc.description.keywords Kinetics
dc.description.keywords Gene Library
dc.description.keywords Alternative Splicing
dc.description.keywords Molecular Sequence Data
dc.description.keywords Baculoviridae
dc.description.keywords DNA, Complementary
dc.description.keywords Receptors, Steroid
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)

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