Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella

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dc.contributor.author Behrendt, L
dc.contributor.author Larkum, AWD
dc.contributor.author Trampe, E
dc.contributor.author Norman, A
dc.contributor.author Sørensen, SJ
dc.contributor.author Kühl, M
dc.date.accessioned 2012-10-12T03:33:33Z
dc.date.issued 2012-06
dc.identifier.citation ISME Journal, 2012, 6 (6), pp. 1222 - 1237
dc.identifier.issn 1751-7362
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18206
dc.description.abstract We assessed the microbial diversity and microenvironmental niche characteristics in the didemnid ascidian Lissoclinum patella using 16S rRNA gene sequencing, microsensor and imaging techniques. L. patella harbors three distinct microbial communities spatially separated by few millimeters of tunic tissue: (i) a biofilm on its upper surface exposed to high irradiance and O 2 levels, (ii) a cloacal cavity dominated by the prochlorophyte Prochloron spp. characterized by strong depletion of visible light and a dynamic chemical microenvironment ranging from hyperoxia in light to anoxia in darkness and (iii) a biofilm covering the underside of the animal, where light is depleted of visible wavelengths and enriched in near-infrared radiation (NIR). Variable chlorophyll fluorescence imaging demonstrated photosynthetic activity, and hyperspectral imaging revealed a diversity of photopigments in all microhabitats. Amplicon sequencing revealed the dominance of cyanobacteria in all three layers. Sequences representing the chlorophyll d containing cyanobacterium Acaryochloris marina and anoxygenic phototrophs were abundant on the underside of the ascidian in shallow waters but declined in deeper waters. This depth dependency was supported by a negative correlation between A. marina abundance and collection depth, explained by the increased attenuation of NIR as a function of water depth. The combination of microenvironmental analysis and fine-scale sampling techniques used in this investigation gives valuable first insights into the distribution, abundance and diversity of bacterial communities associated with tropical ascidians. In particular, we show that microenvironments and microbial diversity can vary significantly over scales of a few millimeters in such habitats; which is information easily lost by bulk sampling. © 2012 International Society for Microbial Ecology All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1038/ismej.2011.181
dc.title Microbial diversity of biofilm communities in microniches associated with the didemnid ascidian Lissoclinum patella
dc.type Journal Article
dc.description.version Published
dc.parent ISME Journal
dc.journal.volume 6
dc.journal.volume 6
dc.journal.number 6 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 1222 en_US
dc.identifier.endpage 1237 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0502 Environmental Science and Management
dc.for 0608 Zoology
dc.for 0605 Microbiology
dc.personcode 111269
dc.personcode 107129
dc.percentage 34 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Acaryochloris marina
dc.description.keywords Lissoclinum patella
dc.description.keywords microbial diversity
dc.description.keywords microenvironment
dc.description.keywords microsensor
dc.description.keywords Prochloron
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)

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