Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms

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dc.contributor.author Staal, M
dc.contributor.author Borisov, SM
dc.contributor.author Rickelt, LF
dc.contributor.author Klimant, I
dc.contributor.author Kühl, M
dc.date.accessioned 2012-10-12T03:34:07Z
dc.date.issued 2011-04
dc.identifier.citation Journal of Microbiological Methods, 2011, 85 (1), pp. 67 - 74
dc.identifier.issn 0167-7012
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18434
dc.description.abstract New transparent optodes for life-time based microscopic imaging of O2 were developed by spin-coating a μm-thin layer of a highly luminescent cyclometalated iridium(III) coumarin complex in polystyrene onto glass cover slips. Compared to similar thin-film O2 optodes based on a ruthenium(II) polypyridyl complex or a platinum(II) porphyrin, the new planar sensors have i) higher brightness allowing for much shorter exposure times and thus higher time resolution, ii) more homogeneous and smaller pixel to pixel variation over the sensor area resulting in less noisy O2 images, and iii) a lower temperature dependency simplifying calibration procedures. We used the new optodes for microscopic imaging of the spatio-temporal O2 dynamics at the base of heterotrophic biofilms in combination with confocal imaging of bacterial biomass and biofilm structure. This allowed us to directly link biomass distribution to O2 distribution under both steady state and non-steady state conditions. We demonstrate that the O2 dynamics in biofilms is governed by a complex interaction between biomass distribution, mass transfer and flow that cannot be directly inferred from structural information on biomass distribution alone. © 2010 Elsevier B.V.
dc.language eng
dc.relation.isbasedon 10.1016/j.mimet.2011.01.021
dc.title Ultrabright planar optodes for luminescence life-time based microscopic imaging of O2 dynamics in biofilms
dc.type Journal Article
dc.description.version Published
dc.parent Journal of Microbiological Methods
dc.journal.volume 1
dc.journal.volume 85
dc.journal.number 1 en_US
dc.publocation Amsterdam en_US
dc.identifier.startpage 67 en_US
dc.identifier.endpage 74 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 107129
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Biofilm
dc.description.keywords Imaging
dc.description.keywords Luminescence
dc.description.keywords Microscopy
dc.description.keywords Optode
dc.description.keywords Oxygen
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true


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