Dysregulation of the inflammatory response to the parasite, Toxoplasma gondii, in P2X7 receptor-deficient mice

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dc.contributor.author Miller, CM
dc.contributor.author Zakrzewski, AM
dc.contributor.author Ikin, RJ
dc.contributor.author Boulter, N
dc.contributor.author Katrib, M
dc.contributor.author Lees, MP
dc.contributor.author Fuller, S
dc.contributor.author Wiley, JS
dc.contributor.author Smith, NF
dc.date.accessioned 2012-10-12T03:34:20Z
dc.date.issued 2011-01
dc.identifier.citation International Journal For Parasitology, 2011, 41 (3-4), pp. 301 - 308
dc.identifier.issn 0020-7519
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/18531
dc.description.abstract The P2X(7) receptor (P2X(7)R) is a two transmembrane receptor that is highly expressed on the surface of immune cells. Loss of function polymorphisms in this receptor have been linked to increased susceptibility to intracellular pathogens. P2X7R gene knockout (P2X(7)R(-/-); on a C57Bl/6J background), C57Bl/6J and BALB/c mice were infected with the avirulent ME49 strain of the intracellular parasite, Toxoplasma gondii. and susceptibility determined by monitoring weight loss. P2X7R(-/-) mice lost significantly more weight than C57Bl/6J mice from day 8 p.i. C57Bl/6J, in turn, lost significantly more weight than BALB/c mice. Thus, by day 10 p.i., P2X(7)R(-/-) mice had lost 5.7 +/- 0.7% of their weight versus 2.4 +/- 0.6% for C57Bl/6J mice, whereas BALB/c mice had gained 1.9 +/- 0.5%; by day 12 p.i., P2X(7)R(-/-) mice had lost 15.1 +/- 0.6%, C57Bl/6J had lost 10.1 +/- 0.8% and BALB/c had lost 4.8 +/- 0.8% of their weight. Neither parasite burden nor liver pathology was greater in the P2X(7)R(-/-) mice than in C57Bl/6J mice but BALB/c mice had significantly smaller numbers of parasites and less pathology in their livers than these strains. Absence of the P2X7 receptor did not affect IFN-gamma, IL-12, IL-1 beta, monocyte chemoattractant protein-1 (MCP-1) or TNF production. However, both P2X(7)R(-/-) and C57Bl/6J mice produced more IL-1 beta and INF than BALB/c mice. There was one important point of differentiation between the P2X(7)R(-/-) and C57Bl/6J mice, namely the significantly enhanced and prolonged production of nitric oxide, accompanied by delayed production of IL-10 in the P2X(7)R-deficient mice.
dc.publisher Elsevier Sci Ltd
dc.relation.isbasedon 10.1016/j.ijpara.2010.10.001
dc.title Dysregulation of the inflammatory response to the parasite, Toxoplasma gondii, in P2X7 receptor-deficient mice
dc.type Journal Article
dc.description.version Published
dc.parent International Journal For Parasitology
dc.journal.volume 3-4
dc.journal.volume 41
dc.journal.number 3-4 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 301 en_US
dc.identifier.endpage 308 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 011172
dc.personcode 044579
dc.personcode 970043
dc.personcode 044726
dc.personcode 103482
dc.personcode 100992
dc.personcode 921144
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity WOS:000288736700005 en_US
dc.description.keywords Phospholipase-D Activation; Nf-Kappa-B; Human Macrophages; Human Monocytes; Cell-Death; Mycobacterium-Tuberculosis; Murine Susceptibility; Signaling Pathways; P2Z Purinoreceptor; Peroral Infection en_US
dc.description.keywords Social Sciences
dc.description.keywords Economics
dc.description.keywords Business & Economics
dc.description.keywords ECONOMICS
dc.description.keywords strategic R & D
dc.description.keywords research joint venture
dc.description.keywords unstable equilibrium
dc.description.keywords SPILLOVERS
dc.description.keywords DUOPOLY
dc.description.keywords Phospholipase-D Activation
dc.description.keywords Nf-Kappa-B
dc.description.keywords Human Macrophages
dc.description.keywords Human Monocytes
dc.description.keywords Cell-Death
dc.description.keywords Mycobacterium-Tuberculosis
dc.description.keywords Murine Susceptibility
dc.description.keywords Signaling Pathways
dc.description.keywords P2Z Purinoreceptor
dc.description.keywords Peroral Infection
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Mathematical Sciences
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)

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