Calcium uptake, respiration, and ultrastructure of sperm exposed to ionophore A23187.

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dc.contributor.author Simpson, AM
dc.contributor.author Swan, MA
dc.contributor.author White, IG
dc.date.accessioned 2014-04-03T01:05:19Z
dc.date.issued 1987
dc.identifier.citation Archives of andrology, 1987, 19 (1), pp. 5 - 18
dc.identifier.issn 0148-5016
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/21958
dc.description.abstract Calcium accumulation by ram sperm in the presence of 3.0 microM ionophore A23187 increased greatly when the pH of the medium was raised from 7.5 to 8.5. The increase in calcium uptake was remarkable between pH 7.5 and 8.0 and was paralleled by increased oxygen consumption. There was extensive vesiculation of the plasma membrane and membranes of the acrosome and postnuclear cap of ram sperm incubated with the ionophore and calcium at pH 8.0. The mitochondria of the midpiece contained pale and expanded cristae similar to ATP-deprived mitochondria in the "condensed" configuration. Such changes were not observed in ram sperm incubated under similar conditions at pH 7.0. The ionophore-induced calcium and oxygen uptake of cauda and caput epididymal boar sperm also increased with increased pH, but the effect commenced at a lower pH. Control cauda epididymal boar sperm (i.e., without ionophore) had higher oxygen uptake, glucose breakdown, and lactic acid production than caput sperm. The influence of pH on the operation of the membrane calcium pumps between ram boar sperm indicates species differences.
dc.format Print
dc.language eng
dc.subject Animals, Sheep, Spermatozoa, Sperm Head, Sperm Tail, Calcium, Calcimycin, Microscopy, Electron, Oxygen Consumption, Sperm Motility, Species Specificity, Hydrogen-Ion Concentration, Swine, Male, Animals, Calcimycin, Calcium, Hydrogen-Ion Concentration, In Vitro Techniques, Male, Microscopy, Electron, Oxygen Consumption, Sheep, Species Specificity, Sperm Head, Sperm Motility, Sperm Tail, Spermatozoa, Swine, Obstetrics & Reproductive Medicine
dc.subject Animals; Sheep; Spermatozoa; Sperm Head; Sperm Tail; Calcium; Calcimycin; Microscopy, Electron; Oxygen Consumption; Sperm Motility; Species Specificity; Hydrogen-Ion Concentration; Swine; Male; Animals; Calcimycin; Calcium; Hydrogen-Ion Concentration; In Vitro Techniques; Male; Microscopy, Electron; Oxygen Consumption; Sheep; Species Specificity; Sperm Head; Sperm Motility; Sperm Tail; Spermatozoa; Swine; Obstetrics & Reproductive Medicine
dc.title Calcium uptake, respiration, and ultrastructure of sperm exposed to ionophore A23187.
dc.type Journal Article
dc.parent Archives of andrology
dc.journal.volume 1
dc.journal.volume 19
dc.journal.number en_US
dc.publocation United States en_US
dc.publocation Perth
dc.identifier.startpage 5 en_US
dc.identifier.endpage 18 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.conference Interior: A state of becoming An IDEA (InteriorDesign/Interior Architecture Educators Association) Symposium
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 940084 en_US
dc.personcode 0000070926 en_US
dc.personcode 0000070925 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.date.activity 2012-09-06
dc.location.activity en_US
dc.location.activity Perth, Australia
dc.description.keywords en_US
dc.description.keywords Spermatozoa
dc.description.keywords Spermatozoa
dc.description.keywords Spermatozoa
dc.description.keywords Sperm Head
dc.description.keywords Sperm Head
dc.description.keywords Sperm Head
dc.description.keywords Sperm Head
dc.description.keywords Sperm Tail
dc.description.keywords Sperm Tail
dc.description.keywords Sperm Tail
dc.description.keywords Sperm Tail
dc.description.keywords Animals
dc.description.keywords Animals
dc.description.keywords Animals
dc.description.keywords Animals
dc.description.keywords Sheep
dc.description.keywords Sheep
dc.description.keywords Sheep
dc.description.keywords Sheep
dc.description.keywords Swine
dc.description.keywords Swine
dc.description.keywords Swine
dc.description.keywords Swine
dc.description.keywords Calcium
dc.description.keywords Calcium
dc.description.keywords Calcium
dc.description.keywords Calcium
dc.description.keywords Calcimycin
dc.description.keywords Calcimycin
dc.description.keywords Calcimycin
dc.description.keywords Calcimycin
dc.description.keywords Microscopy, Electron
dc.description.keywords Microscopy, Electron
dc.description.keywords Microscopy, Electron
dc.description.keywords Microscopy, Electron
dc.description.keywords Sperm Motility
dc.description.keywords Sperm Motility
dc.description.keywords Sperm Motility
dc.description.keywords Sperm Motility
dc.description.keywords Species Specificity
dc.description.keywords Species Specificity
dc.description.keywords Species Specificity
dc.description.keywords Species Specificity
dc.description.keywords Oxygen Consumption
dc.description.keywords Oxygen Consumption
dc.description.keywords Oxygen Consumption
dc.description.keywords Oxygen Consumption
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Male
dc.description.keywords Male
dc.description.keywords Male
dc.description.keywords Male
dc.description.keywords Spermatozoa
dc.description.keywords Spermatozoa
dc.description.keywords Sperm Head
dc.description.keywords Sperm Head
dc.description.keywords Sperm Tail
dc.description.keywords Sperm Tail
dc.description.keywords Animals
dc.description.keywords Animals
dc.description.keywords Sheep
dc.description.keywords Sheep
dc.description.keywords Swine
dc.description.keywords Swine
dc.description.keywords Calcium
dc.description.keywords Calcium
dc.description.keywords Calcimycin
dc.description.keywords Calcimycin
dc.description.keywords Microscopy, Electron
dc.description.keywords Microscopy, Electron
dc.description.keywords Sperm Motility
dc.description.keywords Sperm Motility
dc.description.keywords Species Specificity
dc.description.keywords Species Specificity
dc.description.keywords Oxygen Consumption
dc.description.keywords Oxygen Consumption
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Male
dc.description.keywords Male
dc.description.keywords Animals
dc.description.keywords Sheep
dc.description.keywords Spermatozoa
dc.description.keywords Sperm Head
dc.description.keywords Sperm Tail
dc.description.keywords Calcium
dc.description.keywords Calcimycin
dc.description.keywords Microscopy, Electron
dc.description.keywords Oxygen Consumption
dc.description.keywords Sperm Motility
dc.description.keywords Species Specificity
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Swine
dc.description.keywords Male
dc.description.keywords Animals
dc.description.keywords Sheep
dc.description.keywords Spermatozoa
dc.description.keywords Sperm Head
dc.description.keywords Sperm Tail
dc.description.keywords Calcium
dc.description.keywords Calcimycin
dc.description.keywords Microscopy, Electron
dc.description.keywords Oxygen Consumption
dc.description.keywords Sperm Motility
dc.description.keywords Species Specificity
dc.description.keywords Hydrogen-Ion Concentration
dc.description.keywords Swine
dc.description.keywords Male
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies


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