Label-free impedimetric immunosensor for sensitive detection of 2,4-dichlorophenoxybutyric acid (2,4-DB) in soybean

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dc.contributor.author Zhang, L
dc.contributor.author Wang, M
dc.contributor.author Wang, C
dc.contributor.author Hu, X
dc.contributor.author Wang, G
dc.date.accessioned 2014-04-03T01:22:26Z
dc.date.issued 2012-11-15
dc.identifier.citation Talanta, 2012, 101 pp. 226 - 232
dc.identifier.issn 0039-9140
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/22792
dc.description.abstract Electrochemical impedance immunosensor, with its high sensitivity from electrochemical impedance analysis and ideal specificity from the immunoassay, is increasingly used in the detection of a kind of phenoxy acid herbicides which is 2,4-Dichlorophenoxybutyric acid (2,4-DB). In this experiment, synthetic 2,4-DB antibodies were immobilized on the electrode by the crosslinking of l-Cysteine/glutaraldehyde, and 2,4-DB were measured by the increase of electron-transfer resistance when the immune reaction occurred, with Fe(CN) 63-/Fe(CN)64- as the probe. Under optimal conditions, the change of resistance is in a linear relationship with the logarithm of the concentration in the range of 1.0×10 -7-1.0×10-3 g/L (R=0.994) with the detection limit of 1.0×10-7 g/L (0.1 ppb). This method bears such merits as simplicity in operation, high sensitivity, wide linear range, specificity, reproducibility and good stability. The actual soybean samples were analyzed with the recovery of 82.8%-102.3%. © 2012 Elsevier B.V. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.talanta.2012.09.011
dc.title Label-free impedimetric immunosensor for sensitive detection of 2,4-dichlorophenoxybutyric acid (2,4-DB) in soybean
dc.type Journal Article
dc.parent Talanta
dc.journal.volume 101
dc.journal.number en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 226 en_US
dc.identifier.endpage 232 en_US
dc.cauo.name SCI.School of Chemistry and Forensic Science en_US
dc.conference Verified OK en_US
dc.for 0301 Analytical Chemistry
dc.personcode 109499
dc.personcode 119434
dc.percentage 100 en_US
dc.classification.name Analytical Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Immunosensor; 2,4-DB; l-Cysteine; Glutaraldehyde; Soybean en_US
dc.description.keywords Immunosensor
dc.description.keywords Immunosensor
dc.description.keywords 2,4-DB
dc.description.keywords 2,4-DB
dc.description.keywords l-Cysteine
dc.description.keywords l-Cysteine
dc.description.keywords Glutaraldehyde
dc.description.keywords Glutaraldehyde
dc.description.keywords Soybean
dc.description.keywords Soybean
dc.description.keywords Immunosensor
dc.description.keywords 2,4-DB
dc.description.keywords l-Cysteine
dc.description.keywords Glutaraldehyde
dc.description.keywords Soybean
dc.description.keywords 2,4-DB
dc.description.keywords Glutaraldehyde
dc.description.keywords Immunosensor
dc.description.keywords l-Cysteine
dc.description.keywords Soybean
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group /University of Technology Sydney/Strength - Materials and Technology for Energy Efficiency


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