Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening

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dc.contributor.author Hu, H. en_US
dc.contributor.author Harmer, C. en_US
dc.contributor.author Anuj, S. en_US
dc.contributor.author Wainwright, C. en_US
dc.contributor.author Manos, J. en_US
dc.contributor.author Cheney, J. en_US
dc.contributor.author Harbour, C. en_US
dc.contributor.author Zablotska, I. en_US
dc.contributor.author Grimwood, K. en_US
dc.contributor.author Rose, B. en_US
dc.contributor.author Acfbal Study Investigator, The en_US
dc.contributor.author Turnbull, Lynne en_US
dc.contributor.author Whitchurch, Cynthia en_US
dc.contributor.editor en_US
dc.date.accessioned 2013-11-03T17:05:39Z
dc.date.available 2013-11-03T17:05:39Z
dc.date.issued 2013 en_US
dc.identifier 2012001844 en_US
dc.identifier.citation Hu, H. et al. 2013, 'Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening', Wiley, vol. 19, pp. 266-272. en_US
dc.identifier.issn 1469-0691 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/24058
dc.description.abstract Studies of the type 3 secretion system (T3SS) in Pseudomonas aeruginosa isolates from chronically infected older children and adults with cystic fibrosis (CF) show a predominantly exoS+/exoU) (exoS+) genotype and loss of T3SS effector secretion over time. Relatively little is known about the role of the T3SS in the pathogenesis of early P. aeruginosa infection in the CF airway. In this longitudinal study, 168 P. aeruginosa isolates from 58 children diagnosed with CF following newborn screening and 47 isolates from homes of families with or without children with CF were genotyped by pulsed-field gel electrophoresis (PFGE) and T3SS genotype and phenotype determined using multiplex PCR and western blotting. Associations were sought between T3SS data and clinical variables and comparisons made between T3SS data of clinical and environmental PFGE genotypes. Seventy-seven of the 92 clinical strains were exoS+ (71% secretors (ExoS+)) and 15 were exoU+ (93% secretors (ExoU+)). Initial exoS+ strains were five times more likely to secrete ExoS than subsequent exoS+ strains at first isolation. The proportion of ExoS+ strains declined with increasing age at acquisition. No associations were found between T3SS characteristics and gender, site of isolation, exacerbation, a persistent strain or pulmonary outcomes. Fourteen of the 23 environmental strains were exoS+ (79% ExoS+) and nine were exoU+ (33% ExoU+). The exoU+ environmental strains were significantly less likely to secrete ExoU than clinical strains. This study provides new insight into the T3SS characteristics of P. aeruginosa isolated from the CF airway early in life. en_US
dc.language en_US
dc.publisher Wiley en_US
dc.relation.isbasedon en_US
dc.title Type 3 secretion system effector genotype and secretion phenotype of longitudinally collected Pseudomonas aeruginosa isolates from young children diagnosed with cystic fibrosis following newborn screening en_US
dc.parent Clinical Microbiology and Infection en_US
dc.journal.volume 19 en_US
dc.journal.number en_US
dc.publocation Germany en_US
dc.identifier.startpage 266 en_US
dc.identifier.endpage 272 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 110300 en_US
dc.personcode 0000075733 en_US
dc.personcode 0000085727 en_US
dc.personcode 0000085728 en_US
dc.personcode 0000085729 en_US
dc.personcode 0000085730 en_US
dc.personcode 0000085731 en_US
dc.personcode 0000085732 en_US
dc.personcode 0000085733 en_US
dc.personcode 0000085734 en_US
dc.personcode 0000085735 en_US
dc.personcode 0000085736 en_US
dc.personcode 103744 en_US
dc.personcode 103745 en_US
dc.percentage 100 en_US
dc.classification.name Clinical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Child; cystic fibrosis; phenotype; Pseudomonas aeruginosa; Type 3 secreting system en_US
dc.staffid 103745 en_US
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10


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