Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry

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dc.contributor.author King, Glenn en_US
dc.contributor.author Escoubas, Pierre en_US
dc.contributor.author Leblanc, Mathieu en_US
dc.contributor.author Palagi, Alexandre en_US
dc.contributor.author Wilson, David en_US
dc.contributor.author Dutertre, Sebastien en_US
dc.contributor.author Koh, Jennifer en_US
dc.contributor.author Nicholson, Graham en_US
dc.contributor.editor en_US
dc.date.accessioned 2013-11-03T17:05:39Z
dc.date.available 2013-11-03T17:05:39Z
dc.date.issued 2013 en_US
dc.identifier 2012001532 en_US
dc.identifier.citation King, Glenn et al. 2013, 'Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry', Elsevier, vol. 80, pp. 292-310. en_US
dc.identifier.issn 1874-3919 en_US
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/24060
dc.description.abstract Spider venoms represent vast sources of bioactive molecules whose diversity remains largely unknown. Indeed, only a small subset of species have been studied out of the ~43,000 extant spider species. The present study investigated inter- and intra-species venom complexity in 18 samples collected from a variety of lethal Australian funnel-web spiders (Mygalomorphae: Hexathelidae: Atracinae) using C4 reversed-phase separation coupled to offline MALDI-TOF mass spectrometry (LC-MALDI-TOF MS). An in-depth investigation focusing on four atracine venoms (male Illawarra wisharti, male and female Hadronyche cerberea, and female Hadronyche infensa Toowoomba) revealed, on average, ~800 peptides in female venoms while male venoms contained ~ 400 peptides, distributed across most HPLC fractions. This is significantly higher than previous estimates of peptide expression in mygalomorph venoms. These venoms also showed distinct intersexual as well as intra- and inter-species variation in peptide masses. Construction of both 3D and 2D contour plots revealed that peptide mass distributions in all 18 venoms were centered around the 3200a??5400 m/z range and to a lesser extent the 6600a??8200 m/z range, consistent with previously described hexatoxins. These findings highlight the extensive diversity of peptide toxins in Australian funnel-web spider venoms that that can be exploited as novel therapeutic and biopesticide lead molecules. en_US
dc.language en_US
dc.publisher Elsevier en_US
dc.relation.isbasedon en_US
dc.title Unravelling the complex venom landscapes of lethal Australian funnel-web spiders (Hexathelidae: Atracinae) using LC-MALDI-TOF mass spectrometry en_US
dc.parent Journal of Proteomics en_US
dc.journal.volume 80 en_US
dc.journal.number en_US
dc.publocation Amsterdam en_US
dc.identifier.startpage 292 en_US
dc.identifier.endpage 310 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 111506 en_US
dc.personcode 0000043010 en_US
dc.personcode 0000069097 en_US
dc.personcode 0000082191 en_US
dc.personcode 0000091324 en_US
dc.personcode 0000091325 en_US
dc.personcode 0000094648 en_US
dc.personcode 114867 en_US
dc.personcode 870145 en_US
dc.percentage 50 en_US
dc.classification.name Toxicology (incl. Clinical Toxicology) en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Atrax species Hadronyche species Illawarra wisharti Spider venom LC-MALDI-TOF mass spectrometry Venom landscapes en_US
dc.staffid 870145 en_US

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