The intracellular oxidation of 2',7'-dichlorofluorescin in murine T lymphocytes.

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dc.contributor.author van Reyk, DM
dc.contributor.author King, NJ
dc.contributor.author Dinauer, MC
dc.contributor.author Hunt, NH
dc.date.accessioned 2009-11-12T04:41:10Z
dc.date.issued 2001-01
dc.identifier.citation Free radical biology & medicine, 2001, 30 (1), pp. 82 - 88
dc.identifier.issn 0891-5849
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/3218
dc.description.abstract Intracellular reactive oxygen species (ROS) production by activated murine T lymphocytes was investigated by analyzing intracellular dichlorofluorescin (DCFH(2)) oxidation in lymph node cells (LNC). An increase in DCFH(2) oxidation in LNC induced by phorbol myristate acetate (PMA) was detected by flow cytometry. It was confirmed that this increase was present in Thy1(+) LNC. We examined the contribution to intracellular DCFH(2) oxidation of ROS released by leukocytes other than T cells present in the LNC suspension. Superoxide dismutase, catalase, and glutathione/glutathione peroxidase inhibited the PMA-induced increase in intracellular DCFH(2) oxidation. Furthermore, PMA failed to elicit DCFH(2) oxidation in LNC isolated from mice lacking a functional NADPH oxidase (gp91(phox) gene knockout mice), but this response could be restored in these cells by the addition of T cell-depleted LNC from wild-type litter mates. This study highlights the necessity for caution in using the DCFH(2) assay to demonstrate specific intracellular ROS production in heterogeneous cell populations. It also suggests that cells other than T cells in lymph node populations may, through production of ROS, influence the intracellular redox state of T lymphocytes.
dc.format Print
dc.language eng
dc.relation.isbasedon 10.1016/s0891-5849(00)00449-4
dc.subject Animals, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Mice, Lymph Nodes, T-Lymphocytes, Fluoresceins, Tetradecanoylphorbol Acetate, Reactive Oxygen Species, NADPH Oxidase, Glutathione Peroxidase, Superoxide Dismutase, Glutathione, Oxidation-Reduction, Catalase, Animals, Catalase, Fluoresceins, Glutathione, Glutathione Peroxidase, Lymph Nodes, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, NADPH Oxidase, Oxidation-Reduction, Reactive Oxygen Species, Superoxide Dismutase, T-Lymphocytes, Tetradecanoylphorbol Acetate, Biochemistry & Molecular Biology
dc.subject Animals; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; Mice; Lymph Nodes; T-Lymphocytes; Fluoresceins; Tetradecanoylphorbol Acetate; Reactive Oxygen Species; NADPH Oxidase; Glutathione Peroxidase; Superoxide Dismutase; Glutathione; Oxidation-Reduction; Catalase; Animals; Catalase; Fluoresceins; Glutathione; Glutathione Peroxidase; Lymph Nodes; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Knockout; NADPH Oxidase; Oxidation-Reduction; Reactive Oxygen Species; Superoxide Dismutase; T-Lymphocytes; Tetradecanoylphorbol Acetate; Biochemistry & Molecular Biology
dc.title The intracellular oxidation of 2',7'-dichlorofluorescin in murine T lymphocytes.
dc.type Journal Article
dc.parent Free radical biology & medicine
dc.journal.volume 1
dc.journal.volume 30
dc.journal.number 1 en_US
dc.publocation Oxford, England en_US
dc.identifier.startpage 82 en_US
dc.identifier.endpage 88 en_US
dc.cauo.name Health Sciences en_US
dc.conference Verified OK en_US
dc.for 110106 Medical Biochemistry: Proteins and Peptides (Incl. Medical Proteomics)
dc.personcode 000788 en_US
dc.percentage 100 en_US
dc.classification.name Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics) en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Lymph Nodes
dc.description.keywords T-Lymphocytes
dc.description.keywords Animals
dc.description.keywords Mice, Inbred BALB C
dc.description.keywords Mice, Inbred C57BL
dc.description.keywords Mice, Knockout
dc.description.keywords Mice
dc.description.keywords Reactive Oxygen Species
dc.description.keywords Tetradecanoylphorbol Acetate
dc.description.keywords Fluoresceins
dc.description.keywords NADPH Oxidase
dc.description.keywords Catalase
dc.description.keywords Glutathione Peroxidase
dc.description.keywords Superoxide Dismutase
dc.description.keywords Glutathione
dc.description.keywords Oxidation-Reduction
dc.description.keywords Animals
dc.description.keywords Mice, Inbred BALB C
dc.description.keywords Mice, Inbred C57BL
dc.description.keywords Mice, Knockout
dc.description.keywords Mice
dc.description.keywords Lymph Nodes
dc.description.keywords T-Lymphocytes
dc.description.keywords Fluoresceins
dc.description.keywords Tetradecanoylphorbol Acetate
dc.description.keywords Reactive Oxygen Species
dc.description.keywords NADPH Oxidase
dc.description.keywords Glutathione Peroxidase
dc.description.keywords Superoxide Dismutase
dc.description.keywords Glutathione
dc.description.keywords Oxidation-Reduction
dc.description.keywords Catalase
dc.staffid 000788 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences


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