The development of marine toxicity identification evaluation (TIE) procedures using the unicellular alga Nitzschia closterium

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Show simple item record Hogan, AC Stauber, JL Pablo, F Adams, MS Lim, RP 2009-12-21T02:29:33Z 2005-05
dc.identifier.citation Archives of Environmental Contamination and Toxicology, 2005, 48 (4), pp. 433 - 443
dc.identifier.issn 0090-4341
dc.identifier.other C1 en_US
dc.description.abstract Unicellular algae are highly sensitive to a wide range of toxicants and have been used extensively in ecotoxicological testing. This, along with their ability to grow in very small test volumes over short test durations, make them ideal test organisms for use in Toxicity Identification Evaluations (TIEs). Despite this, microalgae have not previously been used in marine TIE studies. In this study, the marine diatom Nitzschia closterium was shown to be a highly suitable test organism after modification of the standard test protocol to reduce test volumes to 6 mL and test duration to 48 h. The alga was tolerant to the chemicals used in phase I of the standard USEPA TIE protocol, and physical TIE manipulations had no effect on algal growth. The cation exchange procedure, however, inhibited algal growth, while the anion exchange procedure stimulated growth, making these two procedures unsuitable for use with this species. Of the buffers trialed for the graduated pH procedure, 0.01 M PIPES buffer was found to be suitable for buffering at pH 7 because it maintained the required pH over the duration of the test and did not affect the growth or sensitivity of the algae to one reference toxicant (copper). A trial TIE on a secondary-treated sewage effluent for discharge into coastal waters showed that the developed protocols could successfully be used to identify ammonia as the major toxicant in the effluent. © 2005 Springer Science+Business Media, Inc.
dc.language eng
dc.relation.isbasedon 10.1007/s00244-003-0137-y
dc.title The development of marine toxicity identification evaluation (TIE) procedures using the unicellular alga Nitzschia closterium
dc.type Journal Article
dc.description.version Published
dc.description.version Published
dc.parent Archives of Environmental Contamination and Toxicology
dc.journal.volume 4
dc.journal.volume 48
dc.journal.number 4 en_US
dc.publocation New York, USA en_US
dc.publocation Oxford
dc.identifier.startpage 433 en_US
dc.identifier.endpage 443 en_US DVCRch.Institute for Water & Environmental Resource Mgmnt en_US
dc.conference Verified OK en_US
dc.conference Advances in Fracture Research
dc.for 0602 Ecology
dc.personcode 870336
dc.percentage 100 en_US Ecology en_US
dc.classification.type FOR-08 en_US
dc.custom 1.612 en_US 2001-12-02
dc.location.activity Honolulu
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history School of the Environment (ID: 344)
utslib.collection.history School of the Environment (ID: 344)
utslib.collection.history Closed (ID: 3)

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