Development of an improved rapid enzyme inhibition bioassay with marine and freshwater microalgae using flow cytometry

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Show simple item record Franklin, NM Adams, MS Stauber, JL Lim, RP 2009-12-21T02:29:38Z 2001
dc.identifier.citation Archives of Environmental Contamination and Toxicology, 2001, 40 (4), pp. 469 - 480
dc.identifier.issn 0090-4341
dc.identifier.other C1 en_US
dc.description.abstract A rapid toxicity test based on inhibition of esterase activity in marine and freshwater microalgae (Selenastrum capricornutum, Chlorella sp., Dunaliella tertiolecta, Phaeodactylum tricornutum, Tetraselmis sp., Entomoneis cf. punctulata, Nitzschia cf. paleacea) was developed using flow cytometry. Uptake of fluorescein diacetate (FDA) was optimized for each species by varying the substrate concentration, incubation time, and media pH. Propidium iodide (PI) was utilized to assess membrane integrity. The optimized FDA/PI staining procedure was then used to assess the toxicity of copper in short-term exposures (1-24 h). Esterase activity was a sensitive indicator of copper toxicity in S. capricornutum and E. cf. punctulata. As copper concentrations increased, esterase activity decreased in a concentration-dependent manner. The 3- and 24-h EC50 values (based on mean activity states) were 112 μg Cu L-1 (95% confidence limits 88-143) and 51 μg Cu L-1 (95% confidence limits 38-70) for S. capricornutum and 47 μg Cu L-1 (95% confidence limits 43-51) and 9.1 μg Cu L-1 (95% confidence limits 7.6-11) for E. cf. punctulata, respectively. This enzyme inhibition endpoint showed similar sensitivity to chronic growth rate inhibition in E. cf. punctulata (48-h and 72-h EC50 values of 17 and 18 μg L-1, respectively) but was less sensitive compared to growth for S. capricornutum (48-h and 72-h EC50 values of 4.9 and 7.5 μg L-1, respectively). For the other five species tested, inhibition of FDA fluorescence was relatively insensitive to copper, even at copper concentrations that severely inhibited cell division rate. These short-term bioassays that detect sublethal endpoints may provide a more rapid and cost-effective way of monitoring contaminant impacts in natural waters.
dc.language eng
dc.relation.isbasedon 10.1007/s002440010199
dc.title Development of an improved rapid enzyme inhibition bioassay with marine and freshwater microalgae using flow cytometry
dc.type Journal Article
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.parent Archives of Environmental Contamination and Toxicology
dc.journal.volume 4
dc.journal.volume 40
dc.publocation New York, USA en_US
dc.identifier.startpage 469 en_US
dc.identifier.endpage 480 en_US SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 0602 Ecology
dc.for 050102 Ecosystem Function
dc.personcode 870336
dc.percentage 70 en_US Ecosystem Function en_US
dc.classification.type FOR-08 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history School of the Environment (ID: 344)
utslib.collection.history Closed (ID: 3)

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