Inter-polyp genetic and physiological characterisation of Symbiodinium in an Acropora valida colony

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dc.contributor.author Ulstrup, KE
dc.contributor.author Van Oppen, MJH
dc.contributor.author Kühl, M
dc.contributor.author Ralph, PJ
dc.date.accessioned 2009-12-21T02:30:16Z
dc.date.issued 2007-12
dc.identifier.citation Marine Biology, 2007, 153 (2), pp. 225 - 234
dc.identifier.issn 0025-3162
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3814
dc.description.abstract Corals harbouring genetically mixed communities of endosymbiotic algae (Symbiodinium) often show distribution patterns in accordance with differences in light climate across an individual colony. However, the physiology of these genetically characterised communities is not well understood. Single stranded conformation polymorphism (SSCP) and real time quantitative polymerase chain reaction (qPCR) analyses were used to examine the genetic diversity of the Symbiodinium community in hospite across an individual colony of Acropora valida at the spatial scale of single polyps. The physiological characteristics of the polyps were examined prior to sampling with a combined O2 microelectrode with a fibre-optic microprobe (combined sensor diameter 50-100 μm) enabling simultaneous measurements of O2 concentration, gross photosynthesis rate and photosystem II (PSII) quantum yield at the coral surface as a function of increasing irradiances. Both sun- and shade-adapted polyps were found to harbour either Symbiodinium clade C types alone or clades A and C simultaneously. Polyps were grouped in two categories according to (1) their orientation towardps light, or (2) their symbiont community composition. Physiological differences were not detected between sun- and shade-adapted polyps, but O2 concentration at 1,100 μmol photons m-2 s-1 was higher in polyps that harboured both clades A and C symbionts than in polyps that harboured clade C only. These results suggest that the acclimatisation of zooxanthellae of individual polyps of an A. valida colony to ambient light levels may not be the only determinant of the photosynthetic capacity of zooxanthellae. Here, we found that photosynthetic capacity is also likely to have a strong genetic basis and differs between genetically distinct Symbiodinium types. © 2007 Springer-Verlag.
dc.language eng
dc.relation.isbasedon 10.1007/s00227-007-0806-x
dc.title Inter-polyp genetic and physiological characterisation of Symbiodinium in an Acropora valida colony
dc.type Journal Article
dc.parent Marine Biology
dc.journal.volume 2
dc.journal.volume 153
dc.journal.number 2 en_US
dc.publocation Germany en_US
dc.identifier.startpage 225 en_US
dc.identifier.endpage 234 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 0602 Ecology
dc.personcode 890085
dc.personcode 107129
dc.percentage 100 en_US
dc.classification.name Ecology en_US
dc.classification.type FOR-08 en_US
dc.description.keywords acclimation; adaptation; alga; community composition; coral; endosymbiont; genetic analysis; irradiance; photosynthesis; physiology; polymerase chain reaction; polyp; real time; zooxanthella; Acropora valida; algae; Anthozoa; Symbiodinium en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - C3


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