Changes in gene expression associated with stable drug and radiation resistance in small cell lung cancer cells are similar to those caused by a single X-ray dose

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dc.contributor.author Henness, S
dc.contributor.author Davey, MW
dc.contributor.author Harvie, RM
dc.contributor.author Banyer, J
dc.contributor.author Wasinger, V
dc.contributor.author Corthals, G
dc.contributor.author Davey, RA
dc.date.accessioned 2009-12-21T02:30:18Z
dc.date.issued 2004-01
dc.identifier.citation Radiation Research, 2004, 161 pp. 495 - 503
dc.identifier.issn 0033-7587
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3825
dc.description.abstract Small cell lung cancer (SCLC) initially responds well to chemotherapy and fractionated radiotherapy, but resistance to these treatments eventually develops in the vast majority of cases. To understand how resistance develops in the H69 SCLC cell line, we compared the changes in gene expression associated with 37.5 Gy fractionated X-ray treatment that produced the stable radiation- and drug-resistant H69/R38 cell subline to the changes associated with a single 4- or 8-Gy X-ray treatment. Gene expression was determined by suppression subtractive hybridization combined with Northern blot analysis and two-dimensional (2D) protein electrophoresis. Stable radiation and drug resistance was associated with coordinate changes in the expression of genes of the cytoskeleton, protein synthesis, cell cycle, redox/stress and metabolic pathways. The pattern of these changes was remarkably similar to the changes seen 24 h after a single X-ray treatment of the H69 cells but differed from the changes in expression associated with a single X-ray treatment of the resistant H69/ R38 cells. Stable radiation and drug resistance may be caused by the constitutive expression of those genes transiently expressed by sensitive cells in response to a single X-ray dose. The repeated treatments received during fractionated irradiation may promote the change from a transient to a constitutive pattern of gene expression.
dc.publisher Radiation Research Soc
dc.relation.isbasedon 10.1667/RR3165
dc.title Changes in gene expression associated with stable drug and radiation resistance in small cell lung cancer cells are similar to those caused by a single X-ray dose
dc.type Journal Article
dc.parent Radiation Research
dc.journal.volume 161
dc.journal.number en_US
dc.publocation Oak Brook en_US
dc.identifier.startpage 495 en_US
dc.identifier.endpage 503 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060199 Biochemistry and Cell Biology Not Elsewhere Classified
dc.personcode 100152
dc.personcode 880134
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology not elsewhere classified en_US
dc.classification.type FOR-08 en_US
dc.description.keywords NA en_US
dc.description.keywords Humans
dc.description.keywords Carcinoma, Non-Small-Cell Lung
dc.description.keywords Lung Neoplasms
dc.description.keywords Neoplasm Metastasis
dc.description.keywords Tomography, Emission-Computed
dc.description.keywords Neoplasm Staging
dc.description.keywords Adult
dc.description.keywords Aged
dc.description.keywords Middle Aged
dc.description.keywords Female
dc.description.keywords NA
dc.description.keywords NA
dc.description.keywords NA
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10


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