Alternative activation of ruminant macrophages by Fasciola hepatica.

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Show simple item record Flynn, RJ Irwin, JA Olivier, M Sekiya, M Dalton, JP Mulcahy, G 2009-12-21T02:30:36Z 2007-11
dc.identifier.citation Veterinary immunology and immunopathology, 2007, 120 (1-2), pp. 31 - 40
dc.identifier.issn 0165-2427
dc.identifier.other C1 en_US
dc.description.abstract The helminth parasite, Fasciola hepatica, has a worldwide distribution and infects a wide variety of mammalian hosts, including ruminants and man. In response to infection, these hosts mount a type 2 helper (Th2) response that is highly polarized and results in the downregulation of type 1 helper (Th1) mechanisms. In a murine macrophage model F. hepatica induces alternative activation of macrophages. These macrophages differ from classically activated cells in that they preferentially use arginase instead of inducible nitric oxide synthase (iNOS) for metabolism of nitrogen. In this study we sought to characterize macrophage phenotype following stimulation of the ovine cell line MOCL7 with recombinant F. hepatica enzymes and crude parasite extracts. An in vitro model using the MOCL7 cell line was established and arginase levels in cells were used to determine the activation status of cells. Stimulation of this cell-line in vitro with F. hepatica products induces alternative activation. We have also found a chitinase-like protein in supernatants which is capable of differentiating alternatively activated from classically activated macrophages.
dc.format Print-Electronic
dc.language eng
dc.relation.isbasedon 10.1016/j.vetimm.2007.07.003
dc.title Alternative activation of ruminant macrophages by Fasciola hepatica.
dc.type Journal Article
dc.parent Veterinary immunology and immunopathology
dc.journal.volume 1-2
dc.journal.volume 120
dc.journal.number 1-2 en_US
dc.publocation Amsterdam en_US
dc.identifier.startpage 31 en_US
dc.identifier.endpage 40 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0707 Veterinary Sciences
dc.personcode 030896
dc.percentage 100 en_US Veterinary Sciences en_US
dc.classification.type FOR-08 en_US
dc.location.activity ISI:000250616400005 en_US
dc.description.keywords Animals
dc.description.keywords Sheep
dc.description.keywords Macrophages
dc.description.keywords Cells, Cultured
dc.description.keywords Arginase
dc.description.keywords Helminth Proteins
dc.description.keywords Macrophage Activation
dc.description.keywords Peroxiredoxins
dc.description.keywords Nitric Oxide
dc.description.keywords Fasciola hepatica
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true

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