Detection of Dientamoeba fragilis in fresh stool specimens using PCR

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Show simple item record Stark, D Beebe, N Marriott, D Ellis, J Harkness, J 2009-12-21T02:30:38Z 2005-01
dc.identifier.citation International Journal for Parasitology, 2005, 35 (1), pp. 57 - 62
dc.identifier.issn 0020-7519
dc.identifier.other C1 en_US
dc.description.abstract Dientamoeba fragilis is a trichomonad parasite that causes human gastrointestinal disease. Currently microscopy is considered to be the gold standard for diagnosis of D. fragilis infection. However, this method is time-consuming and relatively insensitive. A PCR assay based on the small-subunit ribosomal RNA gene of D. fragilis for the specific detection of D. fragilis DNA in fresh unpreserved stool samples was developed. The D. fragilis PCR was positive in 29/31 samples with positive microscopy and did not cross-react with other protozoan parasites. The PCR protocol showed a specificity of 100% and a sensitivity of 93.5% and the entire procedure can be performed in one day. © 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.ijpara.2004.09.003
dc.title Detection of Dientamoeba fragilis in fresh stool specimens using PCR
dc.type Journal Article
dc.description.version Published
dc.parent International Journal for Parasitology
dc.journal.volume 1
dc.journal.volume 35
dc.journal.number 1 en_US
dc.publocation Oxford, England en_US
dc.identifier.startpage 57 en_US
dc.identifier.endpage 62 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 100210
dc.personcode 104964
dc.personcode 100209
dc.personcode 960052
dc.personcode 910945
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.custom 3.092 en_US
dc.description.keywords Diagnosis
dc.description.keywords Dientamoeba fragilis
dc.description.keywords Faeces
dc.description.keywords PCR
dc.description.keywords Ribosomal DNA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)

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