Functional genomics of gam56: Characterisation of the role of a 56 kilodalton sexual stage antigen in oocyst wall formation in Eimeria maxima

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dc.contributor.author Belli, SI
dc.contributor.author Witcombe, D
dc.contributor.author Wallach, MG
dc.contributor.author Smith, NC
dc.date.accessioned 2009-12-21T02:30:42Z
dc.date.issued 2002-12-19
dc.identifier.citation International Journal for Parasitology, 2002, 32 (14), pp. 1727 - 1737
dc.identifier.issn 0020-7519
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3903
dc.description.abstract Gam56 (Mr 56,000) is an antigen found in the sexual (macrogametocyte) stage of the intestinal parasite Eimeria maxima that is implicated in protective immunity. The gene (gam56) encoding this protein was cloned and sequenced. It is a single-copy, intronless gene, that localises to a 1,754 bp transcript, and is first detected at 120 h p.i. The gene predicts two distinct protein domains; a tyrosine-serine rich region, composed of amino acids implicated in oocyst wall formation in Eimeria spp., and a proline-methionine rich region often detected in extensins, protein components of plant cell walls. The tyrosine-serine rich region predicts a secondary structure commonly seen in the structural protein fibroin, a component of the cocoon of the caterpillar Bombyx mori. The inference that gam56 is a structural component of the oocyst wall was confirmed when a specific antibody to gam56 recognised the wall forming bodies in macrogametocytes, and the walls of oocysts and sporocysts. Together, these data identify a developmentally regulated, sexual stage gene in E. maxima that shares primary and secondary structure features in common with intrinsic structural proteins in other parasites such as Schistosoma mansoni and Fasciola hepatica, and other organisms across different phyla, including the caterpillar Bombyx mori. In addition, these findings provide evidence for the molecular mechanisms underlying oocyst wall formation in Eimeria and the role of gametocyte antigens in this process. © 2002 Australian Soceity for Parasitology Inc. Published by Elsevier Science Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/S0020-7519(02)00212-6
dc.title Functional genomics of gam56: Characterisation of the role of a 56 kilodalton sexual stage antigen in oocyst wall formation in Eimeria maxima
dc.type Journal Article
dc.parent International Journal for Parasitology
dc.journal.volume 14
dc.journal.volume 32
dc.journal.number 14 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 333 en_US
dc.identifier.endpage 341 en_US
dc.cauo.name Environmental Sciences en_US
dc.for 100499 Medical Biotechnology Not Elsewhere Classified
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 960087
dc.personcode 011068
dc.personcode 021128
dc.personcode 990003
dc.description.keywords Eimeria
dc.description.keywords Genes
dc.description.keywords Macrogametocyte
dc.description.keywords Oocyst wall
dc.description.keywords Tyrosine
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)


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