Characterisation of the first Australian isolate of Neospora caninum from cattle

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Show simple item record Miller, CMD Quinn, HE Windsor, PA Ellis, JT 2009-12-21T02:30:46Z 2002-10
dc.identifier.citation Australian Veterinary Journal, 2002, 80 (10), pp. 620 - 625
dc.identifier.issn 0005-0423
dc.identifier.other C1 en_US
dc.description.abstract Objective: To isolate Neospora caninum from a congenitally infected calf. Procedure: A calf was obtained from a N caninum infected dam maintained in a dairy herd of Holstein-Friesian cattle located on the south coast of NSW near Nowra. The calf was euthanased and samples collected for serology and pathology. Samples of brain and spinal cord of the calf were homogenised and injected into immunocompromised mice in an attempt to recover protozoa by in vivo culture. Sequential passage of brain homogenate through IFNγPKO mice was performed and tissue culture flasks were inoculated with brain homogenate. Parasites were identified by electron microscopy and DNA sequencing. The antigen profile of the isolate was analysed using Western blotting. Pathogenicity was examined in BALB/c mice and transmission of the parasite during pregnancy was examined in Qs mice. Results: The calf was seropositive for N caninum and histopathological examination of sections of cerebrum identified lesions consistent with a very mild infection with N caninum. The parasites isolated using tissue culture were identified as N caninum, based on the sequence of the ribosomal DNA and electron microscopy. The antigen profile of the new isolate was similar to that of the NC-Liverpool isolate, but quite different from that of Toxoplasma gondii. In BALB/c mice inoculated with the new isolate, severe clinical signs developed in only three of ten infected mice, compared with six of ten mice infected with NC-Liverpool. Mild to moderate nonsuppurative encephalitis was observed in BALB/c mice infected with the new isolate, compared with mice infected with NC-Liverpool, that developed severe nonsuppurative encephalitis. Transplacental transmission of the isolate arising from an acute infection during pregnancy occurred in about 87% of pups. Conclusion: This is the first isolation of bovine Neospora caninum in Australia. This isolate, called NC-Nowra, appears to be a less virulent form and may prove to be a suitable candidate for vaccine development.
dc.language eng
dc.title Characterisation of the first Australian isolate of Neospora caninum from cattle
dc.type Journal Article
dc.description.version Published
dc.parent Australian Veterinary Journal
dc.journal.volume 10
dc.journal.volume 80
dc.journal.number 10 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 1427 en_US
dc.identifier.endpage 1430 en_US Science en_US
dc.personcode 970043
dc.personcode 910945
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)

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