Indicators of biofilm development and activity in constructed wetlands microcosms

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dc.contributor.author Ragusa, SR
dc.contributor.author McNevin, D
dc.contributor.author Qasem, S
dc.contributor.author Mitchell, C
dc.date.accessioned 2009-12-21T02:30:49Z
dc.date.issued 2004-07
dc.identifier.citation Water Research, 2004, 38 (12), pp. 2865 - 2873
dc.identifier.issn 0043-1354
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3938
dc.description.abstract Methods to measure protein, exopolysaccharide, viable cell number and INT reduction activity were tested on biofilm growing in a wastewater batch reactor. They were shown to be meaningful indicators of biofilm growth and correlated well with each other. Protein, exopolysaccharide, viable cells and INT reduction rates increased linearly over time. Viable cell number exhibited strong linear correlations with protein (R2=0.98) and exopolysaccharide (R 2=0.99) while INT reduction rate was somewhat less well correlated (R2=0.90). Our results indicate production rates of 0.91×10-7μg EPS per viable cell and 1.0×10 -7μg protein per viable cell. Protein and polysaccharide specific INT reduction rates decreased by approximately 50%, whereas viable cell specific INT reduction rates decreased by 65% and the protein to polysaccharide ratio stayed relatively constant at between 1.1 and 1.2 as the biofilm developed. Measurement of protein, polysaccharide, viable cells and INT reduction rate at depth within the bioreactor showed that they were concentrated in the top 1cm of the influent end of the reactor and each decreased to a base level within 4.5cm of the inlet. Protein to polysaccharide ratios increased with depth in the reactor and the specific INT reduction rates were maximal at 4.5cm depth. The results indicate that the biomass can take upwards of 100 days to stabilize during batch (fill and draw) operation of subsurface wetlands and that the relative ratios of biomass components remain relatively constant during biofilm growth. Also, it appears that filtration of suspended solids results in biomass concentration at the inlet to the wetland. © 2004 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.watres.2004.03.039
dc.title Indicators of biofilm development and activity in constructed wetlands microcosms
dc.type Journal Article
dc.parent Water Research
dc.journal.volume 12
dc.journal.volume 38
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 2865 en_US
dc.identifier.endpage 2873 en_US
dc.cauo.name DVCRch.Institute for Sustainable Futures en_US
dc.conference Verified OK en_US
dc.for 0907 Environmental Engineering
dc.for 0799 Other Agricultural and Veterinary Sciences
dc.personcode 010821
dc.percentage 50 en_US
dc.classification.name Environmental Engineering en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Batch
dc.description.keywords Biofilm
dc.description.keywords Constructed wetlands
dc.description.keywords INT reduction
dc.description.keywords Polysaccharide
dc.description.keywords Protein
dc.description.keywords Batch
dc.description.keywords Biofilm
dc.description.keywords Constructed wetlands
dc.description.keywords INT reduction
dc.description.keywords Polysaccharide
dc.description.keywords Protein
dc.description.keywords Batch
dc.description.keywords Biofilm
dc.description.keywords Constructed wetlands
dc.description.keywords INT reduction
dc.description.keywords Polysaccharide
dc.description.keywords Protein
dc.description.keywords Batch
dc.description.keywords Biofilm
dc.description.keywords Constructed wetlands
dc.description.keywords INT reduction
dc.description.keywords Polysaccharide
dc.description.keywords Protein
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/DVC (Research)
pubs.organisational-group /University of Technology Sydney/DVC (Research)/Institute For Sustainable Futures
pubs.organisational-group /University of Technology Sydney/Strength - Sustainable Futures


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