Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle

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dc.contributor.author Hall, CA
dc.contributor.author Reichel, MP
dc.contributor.author Ellis, JT
dc.date.accessioned 2009-12-21T02:30:51Z
dc.date.issued 2006-08-31
dc.identifier.citation Veterinary Parasitology, 2006, 140 (1-2), pp. 61 - 68
dc.identifier.issn 0304-4017
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/3948
dc.description.abstract Aim: To determine the performance characteristics of two enzyme-linked immunosorbent assays (ELISAs) manufactured by Institut Pourquier (IP) for the detection of antibodies against Neospora caninum in bovine sera. Methods: Sera from 526 cattle were assayed in two ELISAs (IP) for the detection of anti-N. caninum antibodies. Results from a further ELISA (IDEXX) were used to provide the "gold standard" N. caninum infection status of the cattle and the ELISA results assessed by two-graph receiver operating characteristic (TG-ROC) analysis. Results: TG-ROC analysis suggested changes to one of the IP ELISA protocols, arriving at a cut-off threshold that was different to the one recommended by the manufacturer. With that change, both of the ELISAs performed with high sensitivity and specificity (in excess of 98%) for bovine sera. Conclusions: The analysis of the two IP ELISAs when used on individual bovine sera demonstrated high sensitivity and specificity. TG-ROC analyses optimised the cut-off point suggested by the manufacturer for one of these commercial diagnostic assays and found agreement with the manufacturer's cut-off regarding the other assay. This will help with the accurate identification of infected animals and thereby contributing to the control of neosporosis. © 2006 Elsevier B.V. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.vetpar.2006.03.016
dc.title Performance characteristics and optimisation of cut-off values of two enzyme-linked immunosorbent assays for the detection of antibodies to Neospora caninum in the serum of cattle
dc.type Journal Article
dc.description.version Published
dc.parent Veterinary Parasitology
dc.journal.volume 1-2
dc.journal.volume 140
dc.journal.number 1-2 en_US
dc.publocation Amsterdam, The Netherlands en_US
dc.identifier.startpage 61 en_US
dc.identifier.endpage 68 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 970072
dc.personcode 105509
dc.personcode 910945
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Cattle
dc.description.keywords ELISA
dc.description.keywords Neospora caninum
dc.description.keywords Protozoa
dc.description.keywords ROC analysis
dc.description.keywords Serology
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Business
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)


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