The effect of surface chemistry modification of titanium alloy on signalling pathways in human osteoblasts

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dc.contributor.author Zreiqat, H
dc.contributor.author Valenzuela, SM
dc.contributor.author Nissan, BB
dc.contributor.author Roest, R
dc.contributor.author Knabe, C
dc.contributor.author Radlanski, RJ
dc.contributor.author Renz, H
dc.contributor.author Evans, PJ
dc.date.accessioned 2009-12-21T02:32:34Z
dc.date.issued 2005-12
dc.identifier.citation Biomaterials, 2005, 26 (36), pp. 7579 - 7586
dc.identifier.issn 0142-9612
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/4296
dc.description.abstract Establishing and maintaining mature bone at the bone-device interface is critical to the long-term success of prosthesis. Poor cell adhesion to orthopaedic and dental implants results in implant failure. Considerable effort has been devoted to alter the surface characteristics of these biomaterials in order to improve the initial interlocking of the device and skeleton. We investigated the effect of surface chemistry modification of titanium alloy (Ti-6Al-4V) with zinc, magnesium or alkoxide-derived hydroxy carbonate apatite (CHAP) on the regulation of key intracellular signalling proteins in human bone-derived cells (HBDC) cultured on these modified Ti-6Al-4V surfaces. Western blotting demonstrated that modifying Ti-6Al-4V with CHAP or Mg results in modulation of key intracellular signalling proteins. We showed an enhanced activation of Shc, a common point of integration between integrins and the Ras/Mapkinase pathway. Mapkinase pathway was also upregulated, suggesting its role in mediating osteoblastic cell interactions with biomaterials. The signalling pathway involving c-fos (member of the activated protein-1) was also shown to be upregulated in osteoblasts cultured on the Mg and CHAP modified Ti-6Al-4V. Thus surface modification with CHAP or Mg may contribute to successful osteoblast function and differentiation at the skeletal tissue-device interface. © 2005 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.biomaterials.2005.05.024
dc.subject Hydroxyapatite, Intracellular signalling proteins, Osteoblasts, Sol-gel coatings, Titanium alloy, Humans, Bone and Bones, Osteoblasts, Cells, Cultured, Apatites, Hydroxyapatites, Magnesium, Zinc, Titanium, Alloys, Extracellular Signal-Regulated MAP Kinases, Proto-Oncogene Proteins c-fos, Biocompatible Materials, Blotting, Western, Osseointegration, Signal Transduction, Phosphorylation, MAP Kinase Signaling System, Up-Regulation, Ions, Integrins, Microscopy, Electron, Scanning, Carbonates, Cell Adhesion, Phase Transition, Surface Properties, Time Factors, Alloys, Apatites, Biocompatible Materials, Blotting, Western, Bone and Bones, Carbonates, Cell Adhesion, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases, Humans, Hydroxyapatites, Integrins, Ions, MAP Kinase Signaling System, Magnesium, Microscopy, Electron, Scanning, Osseointegration, Osteoblasts, Phase Transition, Phosphorylation, Proto-Oncogene Proteins c-fos, Signal Transduction, Surface Properties, Time Factors, Titanium, Up-Regulation, Zinc, hydroxyapatite, sol-gel coatings, titanium alloy, intracellular signalling proteins, osteoblasts, Biomedical Engineering
dc.subject Hydroxyapatite; Intracellular signalling proteins; Osteoblasts; Sol-gel coatings; Titanium alloy; Humans; Bone and Bones; Osteoblasts; Cells, Cultured; Apatites; Hydroxyapatites; Magnesium; Zinc; Titanium; Alloys; Extracellular Signal-Regulated MAP Kinases; Proto-Oncogene Proteins c-fos; Biocompatible Materials; Blotting, Western; Osseointegration; Signal Transduction; Phosphorylation; MAP Kinase Signaling System; Up-Regulation; Ions; Integrins; Microscopy, Electron, Scanning; Carbonates; Cell Adhesion; Phase Transition; Surface Properties; Time Factors; Alloys; Apatites; Biocompatible Materials; Blotting, Western; Bone and Bones; Carbonates; Cell Adhesion; Cells, Cultured; Extracellular Signal-Regulated MAP Kinases; Humans; Hydroxyapatites; Integrins; Ions; MAP Kinase Signaling System; Magnesium; Microscopy, Electron, Scanning; Osseointegration; Osteoblasts; Phase Transition; Phosphorylation; Proto-Oncogene Proteins c-fos; Signal Transduction; Surface Properties; Time Factors; Titanium; Up-Regulation; Zinc; hydroxyapatite; sol-gel coatings; titanium alloy; intracellular signalling proteins; osteoblasts; Biomedical Engineering
dc.title The effect of surface chemistry modification of titanium alloy on signalling pathways in human osteoblasts
dc.type Journal Article
dc.parent Biomaterials
dc.journal.volume 36
dc.journal.volume 26
dc.journal.number 36 en_US
dc.publocation Oxford, England en_US
dc.identifier.startpage 7579 en_US
dc.identifier.endpage 7586 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0904 Chemical Engineering
dc.for 0912 Materials Engineering
dc.personcode 0000024550 en_US
dc.personcode 010690 en_US
dc.personcode 760028 en_US
dc.personcode 101355 en_US
dc.personcode 0000024552 en_US
dc.personcode 0000024553 en_US
dc.personcode 0000024554 en_US
dc.personcode X000089 en_US
dc.percentage 50 en_US
dc.classification.name Materials Engineering en_US
dc.classification.type FOR-08 en_US
dc.custom 3.799 en_US
dc.description.keywords hydroxyapatite; sol-gel coatings; titanium alloy; intracellular signalling proteins; osteoblasts en_US
dc.description.keywords hydroxyapatite
dc.description.keywords sol-gel coatings
dc.description.keywords titanium alloy
dc.description.keywords intracellular signalling proteins
dc.description.keywords osteoblasts
dc.description.keywords Humans
dc.description.keywords Bone and Bones
dc.description.keywords Osteoblasts
dc.description.keywords Cells, Cultured
dc.description.keywords Apatites
dc.description.keywords Hydroxyapatites
dc.description.keywords Magnesium
dc.description.keywords Zinc
dc.description.keywords Titanium
dc.description.keywords Alloys
dc.description.keywords Extracellular Signal-Regulated MAP Kinases
dc.description.keywords Proto-Oncogene Proteins c-fos
dc.description.keywords Biocompatible Materials
dc.description.keywords Blotting, Western
dc.description.keywords Osseointegration
dc.description.keywords Signal Transduction
dc.description.keywords Phosphorylation
dc.description.keywords MAP Kinase Signaling System
dc.description.keywords Up-Regulation
dc.description.keywords Ions
dc.description.keywords Integrins
dc.description.keywords Microscopy, Electron, Scanning
dc.description.keywords Carbonates
dc.description.keywords Cell Adhesion
dc.description.keywords Phase Transition
dc.description.keywords Surface Properties
dc.description.keywords Time Factors
dc.description.keywords Hydroxyapatite
dc.description.keywords Intracellular signalling proteins
dc.description.keywords Osteoblasts
dc.description.keywords Sol-gel coatings
dc.description.keywords Titanium alloy
dc.description.keywords Hydroxyapatite
dc.description.keywords Intracellular signalling proteins
dc.description.keywords Osteoblasts
dc.description.keywords Sol-gel coatings
dc.description.keywords Titanium alloy
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies


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