The effect of surface chemistry modification of titanium alloy on signalling pathways in human osteoblasts

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Show simple item record Zreiqat, H Valenzuela, SM Nissan, BB Roest, R Knabe, C Radlanski, RJ Renz, H Evans, PJ 2009-12-21T02:32:34Z 2005-12
dc.identifier.citation Biomaterials, 2005, 26 (36), pp. 7579 - 7586
dc.identifier.issn 0142-9612
dc.identifier.other C1 en_US
dc.description.abstract Establishing and maintaining mature bone at the bone-device interface is critical to the long-term success of prosthesis. Poor cell adhesion to orthopaedic and dental implants results in implant failure. Considerable effort has been devoted to alter the surface characteristics of these biomaterials in order to improve the initial interlocking of the device and skeleton. We investigated the effect of surface chemistry modification of titanium alloy (Ti-6Al-4V) with zinc, magnesium or alkoxide-derived hydroxy carbonate apatite (CHAP) on the regulation of key intracellular signalling proteins in human bone-derived cells (HBDC) cultured on these modified Ti-6Al-4V surfaces. Western blotting demonstrated that modifying Ti-6Al-4V with CHAP or Mg results in modulation of key intracellular signalling proteins. We showed an enhanced activation of Shc, a common point of integration between integrins and the Ras/Mapkinase pathway. Mapkinase pathway was also upregulated, suggesting its role in mediating osteoblastic cell interactions with biomaterials. The signalling pathway involving c-fos (member of the activated protein-1) was also shown to be upregulated in osteoblasts cultured on the Mg and CHAP modified Ti-6Al-4V. Thus surface modification with CHAP or Mg may contribute to successful osteoblast function and differentiation at the skeletal tissue-device interface. © 2005 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.isbasedon 10.1016/j.biomaterials.2005.05.024
dc.title The effect of surface chemistry modification of titanium alloy on signalling pathways in human osteoblasts
dc.type Journal Article
dc.parent Biomaterials
dc.journal.volume 36
dc.journal.volume 26
dc.journal.number 36 en_US
dc.publocation Oxford, England en_US
dc.identifier.startpage 7579 en_US
dc.identifier.endpage 7586 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0904 Chemical Engineering
dc.for 0912 Materials Engineering
dc.personcode 101355
dc.personcode 760028
dc.personcode 010690
dc.percentage 50 en_US Materials Engineering en_US
dc.classification.type FOR-08 en_US
dc.custom 3.799 en_US
dc.description.keywords Hydroxyapatite
dc.description.keywords Intracellular signalling proteins
dc.description.keywords Osteoblasts
dc.description.keywords Sol-gel coatings
dc.description.keywords Titanium alloy
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)

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