Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation

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dc.contributor.author Peters, PC
dc.contributor.author Thoni, C
dc.contributor.author Harry, L
dc.date.accessioned 2009-12-21T02:32:58Z
dc.date.issued 2006-01
dc.identifier.citation Biotechnic and Histochemistry, 2006, 81 (2-3), pp. 105 - 106
dc.identifier.issn 1052-0295
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/4352
dc.description.abstract A sample of fixed bacterial cells was examined by immunofluorescence microscopy using an Alexa 488 conjugated secondary antibody for visualization. Excitation using visible light confirmed the expected photostability of this fluorophore; however, when us
dc.publisher B I O S Scientific Publishers Ltd
dc.relation.isbasedon 10.1080/10520290600869355
dc.title Unexpected photobleaching of Alexa 488 in a fixed bacterial sample during 2-photon excitation
dc.type Journal Article
dc.parent Biotechnic and Histochemistry
dc.journal.volume 2-3
dc.journal.volume 81
dc.journal.number 2-3 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 105 en_US
dc.identifier.endpage 106 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 1108 Medical Microbiology
dc.personcode 996413
dc.personcode 995003
dc.percentage 100 en_US
dc.classification.name Medical Microbiology en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Alexa 488; bacteria; immunofluorescence microscopy; photobleaching; 2-photon excitation en_US
dc.description.keywords Alexa 488
dc.description.keywords bacteria
dc.description.keywords immunofluorescence microscopy
dc.description.keywords photobleaching
dc.description.keywords 2-photon excitation
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)

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