In vitro dedifferentiation of fetal porcine pancreatic tissue prior to transplantation as islet-like cell clusters.

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dc.contributor.author Humphrey, RK
dc.contributor.author Smith, MS
dc.contributor.author Kwok, J
dc.contributor.author Si, Z
dc.contributor.author Tuch, BE
dc.contributor.author Simpson, AM
dc.date.accessioned 2009-12-21T02:32:58Z
dc.date.issued 2001
dc.identifier.citation Cells, tissues, organs, 2001, 168 (3), pp. 158 - 169
dc.identifier.issn 1422-6405
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/4354
dc.description.abstract The fetal porcine pancreas under experimental conditions can be transplanted in the form of explants or islet-like cell clusters (ICCs) to normalize blood glucose levels in diabetic recipients. ICCs are released from the collagenase-digested pancreas and require a 4- to 5-day culture period for their complete formation. In order to maximize insulin producing beta cell differentiation following transplantation, an understanding of ICC development is essential to utilize this alternative treatment for type 1 diabetes. In this study a role is proposed for exocrine cells in the generation of the multipotent pancreatic precursor cells during the culture period. Acinar cells undergo dedifferentiation during the initial stages of the culture period into multipotent pancreatic precursor cells, previously called protodifferentiated cells. The progressive loss of exocrine differentiation appears to involve rapid degranulation of zymogen granules by exocytosis and loss of the prominent secretory apparatus. These processes occur in parallel with a significant reduction in the expression of lipase in the period from day 0 to day 5 and simultaneously there is an increase in the epithelioid/ductal cell marker, cytokeratin 20. Using proliferating cell nuclear antigen, cell proliferation during the culture period does not appear to account for the increase in epithelioid/ductal cells. Further the rates of apoptosis and necrosis which were identified using the TUNEL technique and propidium iodide, respectively, do not appear to account for the reduction in exocrine cell numbers. Exocrine cell dedifferentiation appears to increase the pool of protodifferentiated cells which have the potential to develop into the insulin-producing beta-cell population following transplantation into the diabetic recipient
dc.format Print
dc.language eng
dc.relation.isbasedon 10.1159/000047831
dc.title In vitro dedifferentiation of fetal porcine pancreatic tissue prior to transplantation as islet-like cell clusters.
dc.type Journal Article
dc.parent Cells, tissues, organs
dc.journal.volume 3
dc.journal.volume 168
dc.journal.number 3 en_US
dc.publocation Switzerland en_US
dc.identifier.startpage 158 en_US
dc.identifier.endpage 169 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 100401 Gene and Molecular Therapy
dc.personcode 940084
dc.percentage 100 en_US
dc.classification.name Gene and Molecular Therapy en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Fetus
dc.description.keywords Animals
dc.description.keywords Pancreas
dc.description.keywords Islets of Langerhans
dc.description.keywords Microscopy, Electron
dc.description.keywords Cell Culture Techniques
dc.description.keywords Islets of Langerhans Transplantation
dc.description.keywords Cell Division
dc.description.keywords Cell Differentiation
dc.description.keywords Cell Aggregation
dc.description.keywords Cell Death
dc.description.keywords Cell Count
dc.description.keywords Pancreatic Ducts
dc.description.keywords Stem Cells
dc.description.keywords Swine
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)


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