Antimony concentrations in nodal tissue can confirm sentinel node identity

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Show simple item record Scolyer, RA Thompson, JF Li, L-XL Beavis, A Dawson, M Doble, P Soper, R Uren, RF Stretch, JR Sharma, R McCarthy, SW 2009-12-21T02:34:29Z 2004-10
dc.identifier.citation Modern Pathology, 2004, 17 (10), pp. 1191 - 1197
dc.identifier.issn 0893-3952
dc.identifier.other C1 en_US
dc.description.abstract The sentinel node biopsy procedure is a highly accurate method of staging patients with cutaneous melanoma and the tumor-harboring status of sentinel nodes is the most important prognostic factor. For the procedure to provide accurate prognostic information, however, it is essential that 'true' sentinel nodes are removed and examined thoroughly. A technique to confirm sentinel node identity may reduce the false-negative rate of the procedure. We have found that antimony (originating from the antimony sulfide colloid used for preoperative lymphoscintigraphy in our institution) can be measured in tissue sections of sentinel nodes using inductively coupled plasma mass spectrometry. The aims of this study were to determine whether antimony concentrations can be used to confirm that removed sentinel nodes are 'true' sentinel nodes and to differentiate sentinel nodes from nonsentinel nodes. In all, 24 patients who had both a tumor-positive sentinel node and a tumor-negative nonsentinel node removed from one regional node field during the same operation, were identified. Tissue sections (50 μm) thick were cut from archival paraffin blocks of each of the sentinel nodes and nonsentinel nodes. Antimony concentrations in the tissue sections were measured using inductively coupled plasma mass spectrometry. The median and mean concentrations of antimony in parts per billion were 0.526 and 1.198, respectively (range 0.020-7.596) in the sentinel nodes, and 0.043 and 0.123 (range 0-0.800) in the nonsentinel nodes (P = 0.004). In four of the 24 pairs, both the presumed sentinel nodes and the nonsentinel nodes had very low antimony levels (less than 0.18 parts per billion), suggesting that nodes designated as sentinel nodes may not have been 'true' sentinel nodes. It is concluded that determination of antimony concentrations within sentinel nodes using the highly sensitive method of inductively coupled plasma mass spectrometry can confirm the identity of sentinel nodes and validate the sentinel node technique.
dc.language eng
dc.relation.isbasedon 10.1038/modpathol.3800202
dc.title Antimony concentrations in nodal tissue can confirm sentinel node identity
dc.type Journal Article
dc.parent Modern Pathology
dc.journal.volume 10
dc.journal.volume 17
dc.journal.number en_US
dc.publocation New York en_US
dc.publocation online
dc.identifier.startpage 1191 en_US
dc.identifier.endpage 1197 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.conference Australian Library and Information Association Conference
dc.for 1103 Clinical Sciences
dc.personcode 910324
dc.personcode 010494
dc.personcode 990445
dc.percentage 100 en_US Clinical Sciences en_US
dc.classification.type FOR-08 en_US 2004-09-21
dc.location.activity Gold Coast, Australia
dc.description.keywords Antimony
dc.description.keywords Mass spectrometry
dc.description.keywords Melanoma
dc.description.keywords Metastases
dc.description.keywords Sentinel node
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Chemistry and Forensic Science
pubs.organisational-group /University of Technology Sydney/Strength - Forensic Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
utslib.collection.history Closed (ID: 3)

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