Rapid assessment of endocrine disruption: vitellogenin (Vtg) expression in male estuarine toadfish (Tetratenos glaber Tetraodontiformes)

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dc.contributor.author Booth, DJ
dc.contributor.author Skene, C
dc.date.accessioned 2009-12-21T02:34:38Z
dc.date.issued 2006-01
dc.identifier.citation Australasian Journal of Ecotoxicology, 2006, 12 (1), pp. 3 - 8
dc.identifier.issn 1323-3475
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/4752
dc.description.abstract Increased contamination of waterways has lead to many impacts on organisms, including effects on reproduction. A suite of endocrine-disruptive chemicals (DECs) has been shown to mimic sex hormones in vertebrates and their presence is an important bioindicator of environmental degradation. We examined expression of vitellogenin (Vtg, a female yolk protein) in male toadfish (Tetratenos glaber), as an indicator of EDC presence in estuaries around Sydney, Australia. First we demonstrated induction of Vtg in males from unpolluted estuarine sites through injection of 17beta-oestradiol. Second, serum of fish from polluted and unpolluted estuaries was collected and examined by reducing-polyacryamide gel electrophoresis (SDS-PAGE). While females from polluted (downstream from sewage treatment plants, and subject to urban runoff) and less polluted sites all expressed Vtg in blood serum, males from less polluted sites showed little or no evidence of Vtg expression. however, most males from heavilty polluted sites showed moderate to high levels of vtg expression indicating that EDCs were present and affecting normal endocrine function in males. We suggest that simple biochemical examinations of EDS effects, such as vtg induction in males, are useful rapid assessment methods wich can provide evidence upon which further, more detailed studies may be undertaken.
dc.publisher Australasian Society for Ecotoxicology
dc.title Rapid assessment of endocrine disruption: vitellogenin (Vtg) expression in male estuarine toadfish (Tetratenos glaber Tetraodontiformes)
dc.type Journal Article
dc.parent Australasian Journal of Ecotoxicology
dc.journal.volume 1
dc.journal.volume 12
dc.journal.number 1 en_US
dc.publocation Cammeray NSW, Australia en_US
dc.publocation Edinburgh UK
dc.identifier.startpage 3 en_US
dc.identifier.endpage 8 en_US
dc.cauo.name DVCRch.Institute for Water & Environmental Resource Mgmnt en_US
dc.conference Verified OK en_US
dc.for 0399 Other Chemical Sciences
dc.personcode 940138
dc.percentage 100 en_US
dc.classification.name Other Chemical Sciences en_US
dc.classification.type FOR-08 en_US
dc.edition 1
dc.description.keywords edocrine-disruptive compounds, estarine fishes, sewage effluent, toadfish (Tetratenos glaber), vitellogenin
dc.description.keywords edocrine-disruptive compounds, estarine fishes, sewage effluent, toadfish (Tetratenos glaber), vitellogenin
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Environmental Science
pubs.organisational-group /University of Technology Sydney/Strength - Environmental Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc true
pubs.consider-herdc true
utslib.collection.history School of the Environment (ID: 344)
utslib.collection.history School of the Environment (ID: 344)
utslib.collection.history Closed (ID: 3)
utslib.collection.history Closed (ID: 3)


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