The antifungal protein from Aspergillus giganteus causes membrane permeabilization

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dc.contributor.author Theis, T
dc.contributor.author Wedde, M
dc.contributor.author Meyer, V
dc.contributor.author Stahl, U
dc.date.accessioned 2009-12-21T02:34:56Z
dc.date.issued 2003-01
dc.identifier.citation Antimicrobial agents and chemotherapy, 2003, 47 (2), pp. 588 - 593
dc.identifier.issn 0066-4804
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/4831
dc.description.abstract We investigated the inhibitory effects of the antifungal protein (AFP) from Aspergillus giganteus on the growth of several filamentous fungi. For this purpose, the MICs of AFP were determined and ranged from 0.1 ?g/ml for Fusarium oxysporum to 200 ?g/ml for Aspergillus nidulans. The antifungal activity of AFP was diminished in the presence of cations. We were able to show that incubation of AFP-sensitive fungi with the protein resulted in membrane permeabilization using an assay based on the uptake of the fluorescent dye SYTOX Green. No permeabilization by AFP could be detected at concentrations below the species-specific MIC. Furthermore, AFP-induced permeabilization could readily be detected after 5 min of incubation. Localization experiments with fluorescein isothiocyanate-labeled AFP and immunofluorescence staining with an AFP-specific antibody supported the observation that the protein interacts with membranes. After treatment of AFP-sensitive fungi with AFP, the protein was localized at the plasma membrane, whereas it was mainly detected inside the cells of AFP-resistant fungi. We conclude from these data that the growth-inhibitory effect of AFP is caused by permeabilization of the fungal membranes.
dc.publisher American Society for Microbiology
dc.relation.isbasedon 10.1128/AAC.47.2.588-593.2003
dc.title The antifungal protein from Aspergillus giganteus causes membrane permeabilization
dc.type Journal Article
dc.parent Antimicrobial agents and chemotherapy
dc.journal.volume 2
dc.journal.volume 47
dc.journal.number 2 en_US
dc.publocation United States en_US
dc.identifier.startpage 588 en_US
dc.identifier.endpage 593 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 060503 Microbial Genetics
dc.for 060501 Bacteriology
dc.personcode 100900
dc.percentage 60 en_US
dc.classification.name Bacteriology en_US
dc.classification.type FOR-08 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)


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