Electrophysiological analysis of the neurotoxic action of a funnel-web spider toxin, δ-atracotoxin-Hv1a, on insect voltage-gated Na+ channels

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dc.contributor.author Grolleau, F
dc.contributor.author Stankiewicz, M
dc.contributor.author Birinyi-Strachan, L
dc.contributor.author Wang, X-H
dc.contributor.author Nicholson, GM
dc.contributor.author Pelhate, M
dc.contributor.author Lapied, B
dc.date.accessioned 2009-12-21T03:52:03Z
dc.date.issued 2001
dc.identifier.citation Journal of Experimental Biology, 2001, 204 (4), pp. 711 - 721
dc.identifier.issn 0022-0949
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/5728
dc.description.abstract The effects of δ-ACTX-Hv1a, purified from the venom of the funnel-web spider Hadronyche versuta, were studied on the isolated giant axon and dorsal unpaired median (DUM) neurones of the cockroach Periplaneta americana under current- and voltage-clamp conditions using the double oil-gap technique for single axons and the patch-clamp technique for neurones. In parallel, the effects of the toxin were investigated on the excitability of rat dorsal root ganglion (DRG) neurones. In both DRG and DUM neurones, δ-ACTX-Hv1a induced spontaneous repetitive firing accompanied by plateau potentials. However, in the case of DUM neurones, plateau action potentials were facilitated when the membrane was artificially hyperpolarized. In cockroach giant axons, δ-ACTX-Hv1a also produced plateau action potentials, but only when the membrane was pre-treated with 3-4 diaminopyridine. Under voltage-clamp conditions, δ-ACTX-Hv1a specifically affected voltage-gated Na+ channels in both axons and DUM neurones. Both the current/voltage and conductance/voltage curves of the δ-ACTX-Hv1a-modified inward current were shifted 10 mV to the left of control curves. In the presence of δ-ACTX-Hv1a, steady-state Na+ channel inactivation became incomplete, causing the appearance of a non-inactivating component at potentials more positive than -40mV. The amplitude of this non-inactivating component was dependent on the holding potential. From this study, it is concluded that, in insect neurones, δ-ACTX-Hv1a mainly affects Na+ channel inactivation by a mechanism that differs slightly from that of scorpion α-toxins.
dc.language eng
dc.title Electrophysiological analysis of the neurotoxic action of a funnel-web spider toxin, δ-atracotoxin-Hv1a, on insect voltage-gated Na+ channels
dc.type Journal Article
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.description.version Published
dc.parent Journal of Experimental Biology
dc.journal.volume 4
dc.journal.volume 204
dc.journal.number en_US
dc.publocation Cambridge, UK en_US
dc.identifier.startpage 711 en_US
dc.identifier.endpage 721 en_US
dc.cauo.name Health Sciences en_US
dc.conference Verified OK en_US
dc.for 060808 Invertebrate Biology
dc.for 030401 Biologically Active Molecules
dc.personcode 870145
dc.percentage 50 en_US
dc.classification.name Biologically Active Molecules en_US
dc.classification.type FOR-08 en_US
dc.description.keywords Atracotoxin
dc.description.keywords Funnel-web spider
dc.description.keywords Hadronyche versuta
dc.description.keywords Insect neurone
dc.description.keywords Na+ channel
dc.description.keywords Spider neurotoxin
dc.description.keywords Vertebrate neurone
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Open Access
utslib.copyright.date 2015-04-15 12:23:47.074767+10
pubs.consider-herdc true


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