Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models

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dc.contributor.author Hare, D
dc.contributor.author Reedy, B
dc.contributor.author Grimm, R
dc.contributor.author Wilkins, S
dc.contributor.author Volitakis, I
dc.contributor.author George, JL
dc.contributor.author Cherny, RA
dc.contributor.author Bush, AI
dc.contributor.author Finkelstein, DI
dc.contributor.author Doble, P
dc.date.accessioned 2010-05-28T09:43:06Z
dc.date.issued 2009-01
dc.identifier.citation Metallomics, 2009, 1 (1), pp. 53 - 58
dc.identifier.issn 1756-5901
dc.identifier.other C1 en_US
dc.identifier.uri http://hdl.handle.net/10453/8417
dc.description.abstract This study demonstrates the application of quantitative elemental bio-imaging for the determination of the distribution Cu, Mn, Fe and Zn in Parkinsonism mouse model brains. Elevated concentrations of these metals within the substantia nigra (SN) are suspected to play a role on the development of Parkinson's disease. Elemental bio-imaging employs laser ablation inductively coupled mass spectrometry (LA-ICP-MS) to construct images of trace element distribution. Quantitative data was produced by ablating the standard tissue sections and recording the mean signal intensity calibrated against multi level matrix matched tissue standards. The concentrations of Fe within the substantia nigra of the lesioned animals increased significantly when compared against control animals. Furthermore, the data was compared against solution nebulisation ICP-MS in which the whole substantia nigra was excised. The trends were the same for both methods; however the elemental bio-imaging method returned significantly higher concentrations. This was caused by dilution from inclusion of surrounding tissue of the SN during the excision procedure. © The Royal Society of Chemistry 2009.
dc.language eng
dc.relation.hasversion Accepted manuscript version en_US
dc.relation.isbasedon 10.1039/b816188g
dc.title Quantitative elemental bio-imaging of Mn, Fe, Cu and Zn in 6-hydroxydopamine induced Parkinsonism mouse models
dc.type Journal Article
dc.parent Metallomics
dc.journal.volume 1
dc.journal.volume 1
dc.journal.number 1 en_US
dc.publocation London, UK en_US
dc.identifier.startpage 53 en_US
dc.identifier.endpage 58 en_US
dc.cauo.name SCI.Chemistry and Forensic Sciences en_US
dc.conference Verified OK en_US
dc.for 039902 Forensic Chemistry
dc.personcode 010494
dc.personcode 000263
dc.personcode 995243
dc.percentage 100 en_US
dc.classification.name Forensic Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Forensic Science
utslib.copyright.status Open Access
utslib.copyright.date 2015-04-15 12:23:47.074767+10
pubs.consider-herdc true
utslib.collection.history General (ID: 2)
utslib.collection.history School of Chemistry and Forensic Science (ID: 339)

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