The development and evaluation of a nested PCR assay for detection of Neospora caninum and Hammondia heydorni in feral mouse tissues

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Show simple item record Barratt, J Al Qassab, S Reichel, MP Ellis, JT 2010-05-28T09:43:18Z 2008-08
dc.identifier.citation Molecular and Cellular Probes, 2008, 22 (4), pp. 228 - 233
dc.identifier.issn 0890-8508
dc.identifier.other C1 en_US
dc.description.abstract The development of a novel nested polymerase chain reaction is described and used for detecting the presence of Neospora caninum and Hammondia heydorni DNA in DNA extracted from feral rodent tissues. A unique strategy was used for design of an assay that could be adapted for detecting DNA from more than one member of Toxoplasmatinae simultaneously with a minimal number of additional steps. The level of sensitivity described for this assay is comparable to real time-PCR and other nested PCR assays. Twenty-eight of 104 feral mice tested positive for N. caninum in at least one tissue (the brain, heart or liver) studied. In this study, eight instances are reported where the brain tested negative to N. caninum while at least one other tissue was positive. This suggests that prior studies, which screened only the brain, describe prevalence levels that are under-represented. None of 54 mouse brains tested positive for H. heydorni DNA. This suggests that mice are rarely infected by H. heydorni although this hypothesis needs to be explored further. Data obtained in the current study suggest that N. caninum is a common parasite of feral rodents which may be important in the epidemiology of the disease. © 2008 Elsevier Ltd. All rights reserved.
dc.language eng
dc.relation.hasversion Accepted manuscript version en_US
dc.relation.isbasedon 10.1016/j.mcp.2008.03.001
dc.title The development and evaluation of a nested PCR assay for detection of Neospora caninum and Hammondia heydorni in feral mouse tissues
dc.type Journal Article
dc.description.version Published
dc.parent Molecular and Cellular Probes
dc.journal.volume 4
dc.journal.volume 22
dc.journal.number en_US
dc.journal.number 4 en_US
dc.publocation UK en_US
dc.identifier.startpage 228 en_US
dc.identifier.endpage 233 en_US SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0304 Medicinal and Biomolecular Chemistry
dc.personcode 998123
dc.personcode 101335
dc.personcode 105509
dc.personcode 910945
dc.percentage 100 en_US Medicinal and Biomolecular Chemistry en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords Hammondia heydorni
dc.description.keywords Mouse
dc.description.keywords Neospora caninum
dc.description.keywords PCR
dc.description.keywords Prevalence
dc.description.keywords Toxoplasmatinae
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Open Access 2015-04-15 12:23:47.074767+10
pubs.consider-herdc true
utslib.collection.history General (ID: 2)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)

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