Distinguishing intra- from extracellular metals: chemical extraction techniques

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dc.contributor.author Hassler, CS
dc.contributor.author Slaveykova, VI
dc.contributor.author Wilkinson, KJ
dc.date.accessioned 2010-05-28T09:43:48Z
dc.date.issued 2004-01
dc.identifier.citation Limnology and Oceanography: Methods, 2004, 2 (1), pp. 237 - 247
dc.identifier.issn 1541-5856
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8531
dc.description.abstract Several washing agents (EDTA, CDTA, NTA, 8 HQS, Ti-EDTA-citrate, Ca2+, and H+) were tested for their ability to extract adsorbed metal from biological surfaces to distinguish between internalized and total cellular metal (Pb, Ni, Cd, Zn, and Cu) for a green alga, Chlorella kesslerii. Although a single extraction protocol cannot be provided that would be acceptable for all biological species and metals, several guidelines are proposed that should facilitate the interpretation of metal bioaccumulation data. It was shown that for the studied metals, a thermodynamic explanation was generally sufficient to explain the metal extraction results. At pH 6.0, a solution of 5 × 103 M ethylenediaminetetraacetic acid (EDTA) was an acceptable washing agent in most cases, whereas Ca or proton exchange solutions did not appear to quantitatively remove adsorbed metals. Conditions of ligand excess could be attained with relatively small concentrations of added ligand, effectively minimizing potential damage to the organism, including modifications in membrane permeability. Potential deleterious effects of the wash agents were verified. For example, a Ti-EDTA-citrate or an EDTA wash at high concentrations resulted in perturbations of algal membrane permeability. In spite of potential difficulties interpreting extraction data, the usefulness of the extraction protocols was clearly demonstrated for several organisms.
dc.publisher American Society of Limnology and Oceanography Inc.
dc.title Distinguishing intra- from extracellular metals: chemical extraction techniques
dc.type Journal Article
dc.parent Limnology and Oceanography: Methods
dc.journal.volume 1
dc.journal.volume 2
dc.journal.number 1 en_US
dc.publocation Waco Texas, USA en_US
dc.identifier.startpage 237 en_US
dc.identifier.endpage 247 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 040502 Chemical Oceanography
dc.personcode 107808
dc.percentage 100 en_US
dc.classification.name Chemical Oceanography en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Open Access
utslib.copyright.date 2015-04-15 12:23:47.074767+10
utslib.collection.history General (ID: 2)

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