Discriminating between intra- and extracellular metals using chemical extractions: an update on the case of iron

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dc.contributor.author Hassler, CS
dc.contributor.author Schoemann, V
dc.date.accessioned 2010-05-28T09:43:51Z
dc.date.issued 2009-01
dc.identifier.citation Limnology and Oceanography: Methods, 2009, 7 pp. 479 - 489
dc.identifier.issn 1541-5856
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8539
dc.description.abstract Iron influences the climate system by limiting primary productivity. It is therefore essential to accurately measure the iron fraction associated with phytoplankton in aquatic systems. A washing procedure using EDTA, being efficient for numerous trace metals, is not strong enough to remove iron adsorbed to the surface of microorganisms. Stronger washing solutions are used for iron, but these have only been assessed for a marine diatom. This study assesses the applicability of the oxalate washing procedure for both fresh- and seawater aquatic systems. We assessed iron solubilization as a result of oxalate washing in both synthetic and natural freshwater and seawater, and we tested it on several model phytoplankton and natural assemblages from Lake Champlain, the Southern Ocean, and the Derwent River estuary. We report the effects of the oxalate solution contact time, concentration, and amendment. Our study shows that 20-min washing provides an efficient measurement of the intracellular phytoplanktonic pool of iron in both freshwater and seawater. The direct amendment of oxalate in the experimental solution presents many advantages that are critical for the measurement of size-fractionated particulate iron. These include fine control of bioaccumulation termination, a significant gain in time, and homogeneity of the washing treatment.
dc.publisher American Society of Limnology and Oceanography Inc.
dc.relation.isbasedon 10.4319/lom.2009.7.479
dc.title Discriminating between intra- and extracellular metals using chemical extractions: an update on the case of iron
dc.type Journal Article
dc.description.version Published
dc.parent Limnology and Oceanography: Methods
dc.journal.volume 7
dc.publocation Waco Texas, USA en_US
dc.identifier.startpage 479 en_US
dc.identifier.endpage 489 en_US
dc.cauo.name SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0602 Ecology
dc.personcode 107808
dc.percentage 100 en_US
dc.classification.name Oceanography en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.description.keywords Science & Technology
dc.description.keywords Life Sciences & Biomedicine
dc.description.keywords Immunology
dc.description.keywords Medicine, Research & Experimental
dc.description.keywords Research & Experimental Medicine
dc.description.keywords IMMUNOLOGY
dc.description.keywords MEDICINE, RESEARCH & EXPERIMENTAL
dc.description.keywords Fasciola hepatica
dc.description.keywords Vaccine
dc.description.keywords Recombinant
dc.description.keywords Cattle
dc.description.keywords SYNTHASE ARGINASE BALANCE
dc.description.keywords NEWLY EXCYSTED JUVENILES
dc.description.keywords IMMUNE-RESPONSES
dc.description.keywords LIVER FLUKE
dc.description.keywords BORDETELLA-PERTUSSIS
dc.description.keywords L PROTEINASES
dc.description.keywords IN-VITRO
dc.description.keywords MACROPHAGES
dc.description.keywords SHEEP
dc.description.keywords ACTIVATION
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Open Access
utslib.copyright.date 2015-04-15 12:23:47.074767+10
utslib.collection.history General (ID: 2)

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