Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa

DSpace/Manakin Repository

Search OPUS


Advanced Search

Browse

My Account

Show simple item record

dc.contributor.author Whitchurch, CB
dc.contributor.author Leech, AJ
dc.contributor.author Young, MD
dc.contributor.author Kennedy, D
dc.contributor.author Sargent, JL
dc.contributor.author Bertrand, JJ
dc.contributor.author Semmler, ABT
dc.contributor.author Mellick, AS
dc.contributor.author Martin, PR
dc.contributor.author Alm, RA
dc.contributor.author Hobbs, M
dc.contributor.author Beatson, SA
dc.contributor.author Huang, B
dc.contributor.author Nguyen, L
dc.contributor.author Commolli, JC
dc.contributor.author Engel, JN
dc.contributor.author Darzins, A
dc.contributor.author Mattick, JS
dc.date.accessioned 2010-05-28T09:44:20Z
dc.date.issued 2004-05
dc.identifier.citation Molecular Microbiology, 2004, 52 (3), pp. 873 - 893
dc.identifier.issn 0950-382X
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8598
dc.description.abstract Virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of a wide range of virulence factors including type IV pili which are required for colonization of host tissues and are associated with a form of surface translocation termed twitching motility. Twitching motility in P. aeruginosa is controlled by a complex signal transduction pathway which shares many modules in common with chemosensory systems controlling flagella rotation in bacteria and which is composed, in part, of the previously described proteins PilG, PilH, Pill, PilJ and PilK. Here we describe another three components of this pathway: ChpA, ChpB and ChpC, as well as two downstream genes, ChpD and ChpE, which may also be involved. The central component of the pathway, ChpA, possesses nine potential sites of phosphorylation: six histidine-containing phosphotransfer (HPt) domains, two novel serine- and threonine-containing phosphotransfer (SPt, TPt) domains and a CheY-like receiver domain at its C-terminus, and as such represents one of the most complex signalling proteins yet described in nature. We show that the Chp chemosensory system controls twitching motillty and type IV pill biogenesis through control of pili assembly and/or retraction as well as expression of the pilin subunit gene pilA. The Chp system is also required for full virulence in a mouse model of acute pneumonia.
dc.language eng
dc.relation.isbasedon 10.1111/j.1365-2958.2004.04026.x
dc.title Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa
dc.type Journal Article
dc.parent Molecular Microbiology
dc.journal.volume 3
dc.journal.volume 52
dc.journal.number 3 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 873 en_US
dc.identifier.endpage 893 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 103745
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000220941400023 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


Files in this item

This item appears in the following Collection(s)

Show simple item record