Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.

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dc.contributor.author Whitchurch, CB
dc.contributor.author Leech, AJ
dc.contributor.author Young, MD
dc.contributor.author Kennedy, D
dc.contributor.author Sargent, JL
dc.contributor.author Bertrand, JJ
dc.contributor.author Semmler, AB
dc.contributor.author Mellick, AS
dc.contributor.author Martin, PR
dc.contributor.author Alm, RA
dc.contributor.author Hobbs, M
dc.contributor.author Beatson, SA
dc.contributor.author Huang, B
dc.contributor.author Nguyen, L
dc.contributor.author Commolli, JC
dc.contributor.author Engel, JN
dc.contributor.author Darzins, A
dc.contributor.author Mattick, JS
dc.date.accessioned 2010-05-28T09:44:20Z
dc.date.issued 2004-05
dc.identifier.citation Molecular microbiology, 2004, 52 (3), pp. 873 - 893
dc.identifier.issn 0950-382X
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8598
dc.description.abstract Virulence of the opportunistic pathogen Pseudomonas aeruginosa involves the coordinate expression of a wide range of virulence factors including type IV pili which are required for colonization of host tissues and are associated with a form of surface translocation termed twitching motility. Twitching motility in P. aeruginosa is controlled by a complex signal transduction pathway which shares many modules in common with chemosensory systems controlling flagella rotation in bacteria and which is composed, in part, of the previously described proteins PilG, PilH, PilI, PilJ and PilK. Here we describe another three components of this pathway: ChpA, ChpB and ChpC, as well as two downstream genes, ChpD and ChpE, which may also be involved. The central component of the pathway, ChpA, possesses nine potential sites of phosphorylation: six histidine-containing phosphotransfer (HPt) domains, two novel serine- and threonine-containing phosphotransfer (SPt, TPt) domains and a CheY-like receiver domain at its C-terminus, and as such represents one of the most complex signalling proteins yet described in nature. We show that the Chp chemosensory system controls twitching motility and type IV pili biogenesis through control of pili assembly and/or retraction as well as expression of the pilin subunit gene pilA. The Chp system is also required for full virulence in a mouse model of acute pneumonia.
dc.format Print
dc.language eng
dc.relation.isbasedon 10.1111/j.1365-2958.2004.04026.x
dc.subject Animals, Mice, Inbred BALB C, Mice, Epithelial Cells, Pseudomonas aeruginosa, Fimbriae, Bacterial, Bacterial Proteins, Fimbriae Proteins, Virulence Factors, Sequence Alignment, Signal Transduction, Multigene Family, Amino Acid Sequence, Molecular Sequence Data, Movement, Amino Acid Sequence, Animals, Bacterial Proteins, Epithelial Cells, Fimbriae Proteins, Fimbriae, Bacterial, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Movement, Multigene Family, Pseudomonas aeruginosa, Sequence Alignment, Signal Transduction, Virulence Factors, Type-4 Fimbrial Biogenesis, Amino-acid-efflux, Bacterial Chemotaxis, Crystal-structure, Iv Pilus, Myxococcus-xanthus, Multistep Phosphorelay, Biofilm Development, Escherichia-coli, Protein-kinases, Microbiology
dc.subject Animals; Mice, Inbred BALB C; Mice; Epithelial Cells; Pseudomonas aeruginosa; Fimbriae, Bacterial; Bacterial Proteins; Fimbriae Proteins; Virulence Factors; Sequence Alignment; Signal Transduction; Multigene Family; Amino Acid Sequence; Molecular Sequence Data; Movement; Amino Acid Sequence; Animals; Bacterial Proteins; Epithelial Cells; Fimbriae Proteins; Fimbriae, Bacterial; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Movement; Multigene Family; Pseudomonas aeruginosa; Sequence Alignment; Signal Transduction; Virulence Factors; Type-4 Fimbrial Biogenesis; Amino-acid-efflux; Bacterial Chemotaxis; Crystal-structure; Iv Pilus; Myxococcus-xanthus; Multistep Phosphorelay; Biofilm Development; Escherichia-coli; Protein-kinases; Microbiology
dc.title Characterization of a complex chemosensory signal transduction system which controls twitching motility in Pseudomonas aeruginosa.
dc.type Journal Article
dc.parent Molecular microbiology
dc.journal.volume 3
dc.journal.volume 52
dc.journal.number 3 en_US
dc.publocation Oxford en_US
dc.identifier.startpage 873 en_US
dc.identifier.endpage 893 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 103745 en_US
dc.personcode 0000047370 en_US
dc.personcode 0000101150 en_US
dc.personcode 0000047371 en_US
dc.personcode 0000047366 en_US
dc.personcode 0000047372 en_US
dc.personcode 0000047373 en_US
dc.personcode 0000051975 en_US
dc.personcode 0000051976 en_US
dc.personcode 0000051977 en_US
dc.personcode 0000047388 en_US
dc.personcode 0000101145 en_US
dc.personcode 0000051981 en_US
dc.personcode 0000047386 en_US
dc.personcode 0000051982 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000220941400023 en_US
dc.location.activity ISI:000220941400023
dc.location.activity ISI:000220941400023
dc.location.activity ISI:000220941400023
dc.description.keywords Type-4 Fimbrial Biogenesis; Amino-acid-efflux; Bacterial Chemotaxis; Crystal-structure; Iv Pilus; Myxococcus-xanthus; Multistep Phosphorelay; Biofilm Development; Escherichia-coli; Protein-kinases en_US
dc.description.keywords Type-4 Fimbrial Biogenesis
dc.description.keywords Type-4 Fimbrial Biogenesis
dc.description.keywords Amino-acid-efflux
dc.description.keywords Amino-acid-efflux
dc.description.keywords Bacterial Chemotaxis
dc.description.keywords Bacterial Chemotaxis
dc.description.keywords Crystal-structure
dc.description.keywords Crystal-structure
dc.description.keywords Iv Pilus
dc.description.keywords Iv Pilus
dc.description.keywords Myxococcus-xanthus
dc.description.keywords Myxococcus-xanthus
dc.description.keywords Multistep Phosphorelay
dc.description.keywords Multistep Phosphorelay
dc.description.keywords Biofilm Development
dc.description.keywords Biofilm Development
dc.description.keywords Escherichia-coli
dc.description.keywords Escherichia-coli
dc.description.keywords Protein-kinases
dc.description.keywords Protein-kinases
dc.description.keywords Type-4 Fimbrial Biogenesis
dc.description.keywords Amino-acid-efflux
dc.description.keywords Bacterial Chemotaxis
dc.description.keywords Crystal-structure
dc.description.keywords Iv Pilus
dc.description.keywords Myxococcus-xanthus
dc.description.keywords Multistep Phosphorelay
dc.description.keywords Biofilm Development
dc.description.keywords Escherichia-coli
dc.description.keywords Protein-kinases
dc.description.keywords Animals
dc.description.keywords Mice, Inbred BALB C
dc.description.keywords Mice
dc.description.keywords Epithelial Cells
dc.description.keywords Pseudomonas aeruginosa
dc.description.keywords Fimbriae, Bacterial
dc.description.keywords Bacterial Proteins
dc.description.keywords Fimbriae Proteins
dc.description.keywords Virulence Factors
dc.description.keywords Sequence Alignment
dc.description.keywords Signal Transduction
dc.description.keywords Multigene Family
dc.description.keywords Amino Acid Sequence
dc.description.keywords Molecular Sequence Data
dc.description.keywords Movement
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3


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