Quorum-sensing regulation of adhesion in Serratia marcescens MG1 is surface dependent.

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dc.contributor.author Labbate, M
dc.contributor.author Zhu, H
dc.contributor.author Thung, L
dc.contributor.author Bandara, R
dc.contributor.author Larsen, MR
dc.contributor.author Willcox, MD
dc.contributor.author Givskov, M
dc.contributor.author Rice, SA
dc.contributor.author Kjelleberg, S
dc.date.accessioned 2010-05-28T09:44:36Z
dc.date.issued 2007-04
dc.identifier.citation Journal of bacteriology, 2007, 189 (7), pp. 2702 - 2711
dc.identifier.issn 0021-9193
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8648
dc.description.abstract Serratia marcescens is an opportunistic pathogen and a major cause of ocular infections. In previous studies of S. marcescens MG1, we showed that biofilm maturation and sloughing were regulated by N-acyl homoserine lactone (AHL)-based quorum sensing (QS). Because of the importance of adhesion in initiating biofilm formation and infection, the primary goal of this study was to determine whether QS is important in adhesion to both abiotic and biotic surfaces, as assessed by determining the degree of attachment to hydrophilic tissue culture plates and human corneal epithelial (HCE) cells. Our results demonstrate that while adhesion to the abiotic surface was AHL regulated, adhesion to the HCE cell biotic surface was not. Type I fimbriae were identified as the critical adhesin for non-QS-mediated attachment to the biotic HCE cell surface but played no role in adhesion to the abiotic surface. While we were not able to identify a single QS-regulated adhesin essential for attachment to the abiotic surface, four AHL-regulated genes involved in adhesion to the abiotic surface were identified. Interestingly, two of these genes, bsmA and bsmB, were also shown to be involved in adhesion to the biotic surface in a non-QS-controlled fashion. Therefore, the expression of these two genes appears to be cocontrolled by regulators other than the QS system for mediation of attachment to HCE cells. We also found that QS in S. marcescens regulates other potential cell surface adhesins, including exopolysaccharide and the outer membrane protein OmpX. We concluded that S. marcescens MG1 utilizes different regulatory systems and adhesins in attachment to biotic and abiotic surfaces and that QS is a main regulatory pathway in adhesion to an abiotic surface but not in adhesion to a biotic surface.
dc.format Print-Electronic
dc.language eng
dc.relation.isbasedon 10.1128/jb.01582-06
dc.title Quorum-sensing regulation of adhesion in Serratia marcescens MG1 is surface dependent.
dc.type Journal Article
dc.parent Journal of bacteriology
dc.journal.volume 7
dc.journal.volume 189
dc.journal.number en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 2702 en_US
dc.identifier.endpage 2711 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 106011
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.location.activity United States
dc.description.keywords Humans
dc.description.keywords Cornea
dc.description.keywords Epithelial Cells
dc.description.keywords Pseudomonas aeruginosa
dc.description.keywords RNA, Bacterial
dc.description.keywords RNA, Ribosomal, 16S
dc.description.keywords Phylogeny
dc.description.keywords Bacterial Adhesion
dc.description.keywords Plasmids
dc.description.keywords Molecular Sequence Data
dc.description.keywords 4-Butyrolactone
dc.description.keywords Polysaccharides, Bacterial
dc.description.keywords Surface Properties
dc.description.keywords Serratia marcescens
dc.description.keywords Quorum Sensing
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history Closed (ID: 3)

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