Recovery of new integron classes from environmental DNA

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Show simple item record Nield, BS Holmes, AJ Gillings, MR Recchia, GD Mabbutt, BC Nevalainen, KMH Stokes, HW 2010-05-28T09:44:37Z 2001-02-05
dc.identifier.citation FEMS Microbiology Letters, 2001, 195 (1), pp. 59 - 65
dc.identifier.issn 0378-1097
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Integrons are genetic elements known for their role in the acquisition and expression of genes conferring antibiotic resistance. Such acquisition is mediated by an integron-encoded integrase, which captures genes that are part of gene cassettes. To test whether integrons occur in environments with no known history of antibiotic exposure, PCR primers were designed to conserved regions of the integrase gene and the gene cassette recombination site. Amplicons generated from four environmental DNA samples contained features typical of the integrons found in antibiotic-resistant and pathogenic bacteria. The sequence diversity of the integrase genes in these clones was sufficient to classify them within three new classes of integron. Since they are derived from environments not associated with antibiotic use, integrons appear to be more prevalent in bacteria than previously observed. © 2001 Federation of European Microbiological Societies.
dc.language eng
dc.relation.isbasedon 10.1016/S0378-1097(00)00546-2
dc.title Recovery of new integron classes from environmental DNA
dc.type Journal Article
dc.parent FEMS Microbiology Letters
dc.journal.volume 1
dc.journal.volume 195
dc.journal.number 1 en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 59 en_US
dc.identifier.endpage 65 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 104673
dc.personcode 105741
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords Environmental DNA
dc.description.keywords Environmental DNA
dc.description.keywords Gene cassette
dc.description.keywords Gene cassette
dc.description.keywords Integron
dc.description.keywords Integron
dc.description.keywords Tyrosine recombinase
dc.description.keywords Tyrosine recombinase
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)

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