Recovery of new integron classes from environmental DNA.

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dc.contributor.author Nield, BS
dc.contributor.author Holmes, AJ
dc.contributor.author Gillings, MR
dc.contributor.author Recchia, GD
dc.contributor.author Mabbutt, BC
dc.contributor.author Nevalainen, KM
dc.contributor.author Stokes, HW
dc.date.accessioned 2010-05-28T09:44:37Z
dc.date.issued 2001-02
dc.identifier.citation FEMS microbiology letters, 2001, 195 (1), pp. 59 - 65
dc.identifier.issn 0378-1097
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8649
dc.description.abstract Integrons are genetic elements known for their role in the acquisition and expression of genes conferring antibiotic resistance. Such acquisition is mediated by an integron-encoded integrase, which captures genes that are part of gene cassettes. To test whether integrons occur in environments with no known history of antibiotic exposure, PCR primers were designed to conserved regions of the integrase gene and the gene cassette recombination site. Amplicons generated from four environmental DNA samples contained features typical of the integrons found in antibiotic-resistant and pathogenic bacteria. The sequence diversity of the integrase genes in these clones was sufficient to classify them within three new classes of integron. Since they are derived from environments not associated with antibiotic use, integrons appear to be more prevalent in bacteria than previously observed.
dc.format Print
dc.language eng
dc.relation.isbasedon 10.1111/j.1574-6968.2001.tb10498.x
dc.subject Integrases, Soil, Polymerase Chain Reaction, Sequence Analysis, DNA, Base Sequence, Plasmids, Amino Acid Sequence, Molecular Sequence Data, Amino Acid Sequence, Base Sequence, Integrases, Molecular Sequence Data, Plasmids, Polymerase Chain Reaction, Sequence Analysis, DNA, Soil, Integron Tyrosine recombinase Gene cassette Environmental DNA, Microbiology
dc.subject Integrases; Soil; Polymerase Chain Reaction; Sequence Analysis, DNA; Base Sequence; Plasmids; Amino Acid Sequence; Molecular Sequence Data; Amino Acid Sequence; Base Sequence; Integrases; Molecular Sequence Data; Plasmids; Polymerase Chain Reaction; Sequence Analysis, DNA; Soil; Integron Tyrosine recombinase Gene cassette Environmental DNA; Microbiology
dc.title Recovery of new integron classes from environmental DNA.
dc.type Journal Article
dc.parent FEMS microbiology letters
dc.journal.volume 1
dc.journal.volume 195
dc.journal.number 1 en_US
dc.publocation Amsterdam, Netherlands en_US
dc.identifier.startpage 59 en_US
dc.identifier.endpage 65 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 104673 en_US
dc.personcode 0000050528 en_US
dc.personcode 0000030144 en_US
dc.personcode 0000021502 en_US
dc.personcode 0000050529 en_US
dc.personcode 0000027049 en_US
dc.personcode 105741 en_US
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords Integron ? Tyrosine recombinase ? Gene cassette ? Environmental DNA en_US
dc.description.keywords Integron Tyrosine recombinase Gene cassette Environmental DNA
dc.description.keywords Integron Tyrosine recombinase Gene cassette Environmental DNA
dc.description.keywords Integron Tyrosine recombinase Gene cassette Environmental DNA
dc.description.keywords Integron Tyrosine recombinase Gene cassette Environmental DNA
dc.description.keywords Integron Tyrosine recombinase Gene cassette Environmental DNA
dc.description.keywords Integrases
dc.description.keywords Soil
dc.description.keywords Polymerase Chain Reaction
dc.description.keywords Sequence Analysis, DNA
dc.description.keywords Amino Acid Sequence
dc.description.keywords Base Sequence
dc.description.keywords Plasmids
dc.description.keywords Molecular Sequence Data
dc.description.keywords Integrases
dc.description.keywords Soil
dc.description.keywords Polymerase Chain Reaction
dc.description.keywords Sequence Analysis, DNA
dc.description.keywords Base Sequence
dc.description.keywords Plasmids
dc.description.keywords Amino Acid Sequence
dc.description.keywords Molecular Sequence Data
dc.staffid 105741 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences


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