The sedimentation equilibrium of heterogeneously associating systems and mixtures of non-interacting solutes: analysis without determination of molecular weight averages

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dc.contributor.author Nichol, LW
dc.contributor.author Jeffrey, PD
dc.contributor.author Milthorpe, BK
dc.date.accessioned 2010-05-28T09:44:42Z
dc.date.issued 1976
dc.identifier.citation Biophysical Chemistry, 1976, 4 (3), pp. 259 - 267
dc.identifier.issn 0301-4622
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8663
dc.description.abstract Sedimentation equilibrium is first considered of a system in which a ligand of any size binds to an acceptor at p sites, the experimental result, obtained with either interference or absorption optics, being a distribution of total solute concentration as a function of radial distance. Theory illustrated by a numerical example, is presented which shows that this distribution may be analysed to give the activity of the unbound ligand as a function of total weight concentration. It is shown that this information may be used together with conservation of mass equations written in terms of the initial mixing composition to evaluate the equilibrium constant(s) relevant to the system. Correlation with composition evaluation by use of absorption optics (when possible) is also discussed. The procedure does not involve solution of simultaneous equations which are sums of exponentials nor differentiation of experimental results to obtain apparent weight-average molecular weights. It is general in that it leads to the evaluation of the activity of the species characterized by the smallest M(l-vπ) product and, accordingly, is shown to be useful in the analysis of non-interacting as well as of interacting systems. © 1976.
dc.language eng
dc.relation.isbasedon 10.1016/0301-4622(76)80073-7
dc.subject Ligands, Kinetics, Binding Sites, Computers, Molecular Weight, Solubility, Mathematics, Thermodynamics, Binding Sites, Computers, Kinetics, Ligands, Mathematics, Molecular Weight, Solubility, Thermodynamics, NA, Biophysics
dc.subject Ligands; Kinetics; Binding Sites; Computers; Molecular Weight; Solubility; Mathematics; Thermodynamics; Binding Sites; Computers; Kinetics; Ligands; Mathematics; Molecular Weight; Solubility; Thermodynamics; NA; Biophysics
dc.title The sedimentation equilibrium of heterogeneously associating systems and mixtures of non-interacting solutes: analysis without determination of molecular weight averages
dc.type Journal Article
dc.parent Biophysical Chemistry
dc.journal.volume 3
dc.journal.volume 4
dc.journal.number 3 en_US
dc.publocation Netherlands en_US
dc.identifier.startpage 259 en_US
dc.identifier.endpage 267 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 0000049736 en_US
dc.personcode 0000049735 en_US
dc.personcode 105631 en_US
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.description.keywords NA
dc.staffid 105631 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies


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