Complementation Analysis in Pseudomonas aeruginosa of the Transfer Genes of the Wide Host Range R Plasmid RI8

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dc.contributor.author Stokes, H
dc.contributor.author Moore, RJ
dc.contributor.author Krishnapillai, V
dc.date.accessioned 2010-05-28T09:44:48Z
dc.date.issued 1981-01
dc.identifier.citation Plasmid, 1981, 5 (2), pp. 202 - 212
dc.identifier.issn 0147-619X
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8679
dc.description.abstract A method of transductional complementation was developed in Pseudomonas aeruginosa to identify the cistrons involved in the conjugal transfer of the wide host range R plasmid R18. This used the P. aeruginosa bacteriophage E79tv-2 and has led to the identification of eight tra cistrons encoded by this plasmid. Plasmids mutant in six cistrons, traA, traB, traC, traD, traE, and traG were resistant to donor-specific phage (Dps-) while traF and traH mutant plasmids retained phage sensitivity. Some traB mutants were unable to inhibit the replication of phage G101 (Phi(G101)-) while some were also deficient in entry exclusion (Eex-). Two traB mutants which were also Eex- were suppressible by an amber suppressor. Three tra mutants selected directly as being Phi(G101)- were found to be also Dps-Eex- and mutant in traB. These data suggest a relationship between traB, Eex, and Phi(G101). In order to facilitate future genetic comparison of the tra genes of R18 and other wide host range plasmids and the role of the host in conjugation, R18 DNA was compared with that of RP4, by restriction enzyme fragment patterns and found to be identical
dc.format No Code
dc.publisher Academic Press
dc.relation.isbasedon 10.1016/0147-619X(81)90021-4
dc.title Complementation Analysis in Pseudomonas aeruginosa of the Transfer Genes of the Wide Host Range R Plasmid RI8
dc.type Journal Article
dc.parent Plasmid
dc.journal.volume 2
dc.journal.volume 5
dc.journal.number 2 en_US
dc.publocation United States en_US
dc.identifier.startpage 202 en_US
dc.identifier.endpage 212 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 105741
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords NA en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)


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