Optimization of Iron dependent cyanobacterial (Synechococcus, Cyanophyceae) bioreporters to measure iron bioavailability

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dc.contributor.author Hassler, CS
dc.contributor.author Twiss, RM
dc.contributor.author McKay, RM
dc.contributor.author Bullerjahn, GS
dc.date.accessioned 2010-05-28T09:44:51Z
dc.date.issued 2006-01
dc.identifier.citation Journal of Phycology, 2006, 42 (2), pp. 324 - 335
dc.identifier.issn 0022-3646
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8688
dc.description.abstract Complex chemistry and biological uptake pathways render iron bioavailability particularly difficult to assess in natural waters. Bioreporters are genetically modified organisms that are useful tools to directly sense the bioavailable fractions of solutes. In this study, three cyanobacterial bioreporters derived from Synechococcus PCC 7942 were examined for the purpose of optimizing the response to bioavailable Fe. Each bioreporter uses a Fe-regulated promoter ( isiAB, irpA and mapA), modulated by distinct mechanisms under Fe deficiency, fused to a bacterial luciferase ( luxAB). In order to provide a better understanding of the way natural conditions may affect the ability of the bioreporter to sense iron bioavailability, the effect of relevant environmental parameters on the response to iron was assessed. Optimal conditions (and limits of applicability) for the use of these bioreporters on the field were determined to be: a 12 h (1224 h) exposure time, temperature of 15°C (15°C22°C), photon flux density of 100 ?mol photons·m<sup>-2</sup>·s<sup>-1</sup> (37200 lmol photons·m<sup>-2</sup>·s<sup>-1</sup>), initial biomass of 0.60.8 lg chlorophyll a (chl a)·L<sup>-1</sup> (0.31.5 lg chl a·L<sup>-1</sup>) or approximately 10<sup>5</sup> bioreporter cells·mL<sup>-1</sup>, high phosphate (10 lM), and low micronutrients (absent).
dc.publisher Blackwell Publishing Ltd
dc.relation.isbasedon 10.1111/j.1529-8817.2006.00203.x
dc.title Optimization of Iron dependent cyanobacterial (Synechococcus, Cyanophyceae) bioreporters to measure iron bioavailability
dc.type Journal Article
dc.parent Journal of Phycology
dc.journal.volume 2
dc.journal.volume 42
dc.journal.number 2 en_US
dc.publocation USA en_US
dc.identifier.startpage 324 en_US
dc.identifier.endpage 335 en_US
dc.cauo.name SCI.Environmental Sciences en_US
dc.conference Verified OK en_US
dc.for 0607 Plant Biology
dc.personcode 107808
dc.percentage 100 en_US
dc.classification.name Plant Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords bioavailability; bioreporter; cyanobacteria; iron; isiAB en_US
dc.description.keywords bioavailability
dc.description.keywords bioreporter
dc.description.keywords cyanobacteria
dc.description.keywords iron
dc.description.keywords isiAB
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)

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