The direct analysis of sedimentation equilibrium results obtained with polymerizing systems

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dc.contributor.author Milthorpe, BK
dc.contributor.author Jeffrey, PD
dc.contributor.author Nichol, LW
dc.date.accessioned 2010-05-28T09:44:58Z
dc.date.issued 1975
dc.identifier.citation Biophysical Chemistry, 1975, 3 (2), pp. 169 - 176
dc.identifier.issn 0301-4622
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8708
dc.description.abstract Theory is presented in relation to sedimentation equilibrium results obtained with polymerizing systems, which permits evaluation of the activity of the monomer as a function of total weight concentration. In contrast to established methods, the suggested procedure does not involve the solution of simultaneous equations which are sums of exponentials or the determination of weight-average molecular weights. A major advantage of the method is that it avoids errors inherent in differentiation and integration steps. An extrapolation to infinite dilution is involved, but this is to a defined limit and is uncomplicated by the existence of critical points in the relevant plot. The method is capable of detecting possible volume changes inherent on polymer formation, of treating systems where activity coefficients of solute species are functions of total concentration and of describing the system in terms of relevant equilibrium constants. These points and comparisons with existing methods of analysis are illustrated with numerical examples and with results obtained with lysozyme at pH 6.7. The lysozyme results are interpretable in terms of either a non-ideal monomer-dimer system or a monomer-dimer-trimer system. © 1975.
dc.language eng
dc.relation.isbasedon 10.1016/0301-4622(75)80007-X
dc.title The direct analysis of sedimentation equilibrium results obtained with polymerizing systems
dc.type Journal Article
dc.parent Biophysical Chemistry
dc.journal.volume 2
dc.journal.volume 3
dc.journal.number 2 en_US
dc.publocation Netherlands en_US
dc.identifier.startpage 169 en_US
dc.identifier.endpage 176 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.personcode 105631
dc.percentage 100 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - Health Technologies
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)


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