Vaccinia virus replication is independent of cellular HSP72 expression which is induced during virus infection

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dc.contributor.author Sedger, LM
dc.contributor.author Ramshaw, I
dc.contributor.author Condie, A
dc.contributor.author Medveczky, J
dc.contributor.author Braithwaite, A
dc.contributor.author Ruby, J
dc.date.accessioned 2010-05-28T09:45:03Z
dc.date.issued 1996-01
dc.identifier.citation Virology, 1996, 225 (2), pp. 423 - 427
dc.identifier.issn 0042-6822
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8722
dc.description.abstract HSP72 is dramatically induced in the ovaries of vaccinia virus (VV)-infected mice and associates with VV proteins. In order to investigate the role of HSP72 during vaccinia virus replication, we have constructed a recombinant vaccinia virus encoding the major inducible cellular HSP72 (VV-HSP72+) and examined the replication characteristics of this virus. VV-HSP72+ exhibited growth kinetics identical to and peak titers very similar to those of control viruses, both in vitro and in vivo. In particular, replication of VV-HSP72+ was identical to that of control viruses in the HSP72-negative cell line Y3.Ag.1.2.3, and overexpression of HSP72 had no effect on the virulence of VV infection in normal or immunocompromised mice. We conclude that while VV infection results in the induction of the major inducible 72-kDa HSP, VV replication proceeds normally in the absence of this protein. It is unclear whether another celluar chaperone is required to facilitate virus replication in place of HSP72 in Y3.Ag.1.2.3 cells or whether HSP expression plays no role in virus replication, but is simply a component of the generalized stress response to virus infection. (C) 1996 Academic Press, Inc.
dc.format Esha Dutt
dc.publisher Academic Press
dc.relation.isbasedon 10.1006/viro.1996.0619
dc.title Vaccinia virus replication is independent of cellular HSP72 expression which is induced during virus infection
dc.type Journal Article
dc.parent Virology
dc.journal.volume 2
dc.journal.volume 225
dc.journal.number 2 en_US
dc.publocation USA en_US
dc.identifier.startpage 423 en_US
dc.identifier.endpage 427 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060103 Cell Development, Proliferation and Death
dc.for 060506 Virology
dc.personcode 102397
dc.percentage 60 en_US
dc.classification.name Virology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:A1996VV42100021 en_US
dc.description.keywords Heat-Shock proteins, beet yellows closterovirus en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)


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