Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol trasnport locus (gtsABC)

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Show simple item record Djordjevic, SP Vilei, EM Frey, J 2010-05-28T09:45:07Z 2003-01
dc.identifier.citation Microbiology-uk, 2003, 149 pp. 195 - 204
dc.identifier.issn 1350-0872
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Mycoplasma species bovine group 7, represented by the type strain PG50, is one of six members of the Mycoplasma mycoides cluster and has been implicated in sporadic and outbreak cases of polyarthritis and mastitis in Australian dairy cattle. This study describes cloning and sequencing a 7·9 kb region of the PG50 chromosome and identification of genes involved in glycerol transport (gtsA, gtsB and gtsC) that are followed by a putative lipoprotein gene lppB and a genomic locus containing two ORFs encoding putative membrane proteins. Long range PCR using primers spanning gtsABC and downstream flanking genes, and Southern hybridization analyses using a suite of probes derived from M. mycoides subsp. mycoides small colony type (SC) strain Afadé for gtsA, gtsB and gtsC, lppB and the two downstream genes confirmed that these genes were conserved among Mycoplasma sp. bovine group 7 isolates and mycoplasmas belonging to the M. mycoides subcluster [M. mycoides subsp. mycoides SC, M. mycoides subsp. mycoides large colony type (LC) and M. mycoides subsp. capri] but were absent in mycoplasmas belonging to the Mycoplasma capricolum subcluster (M. capricolum subsp. capricolum and M. capricolum subsp. capripneumoniae). M. capricolum subsp. capricolum type strain California kid did not hybridize with the probe for gtsA and gave only weak or no hybridization signals with probes derived from the loci downstream of gtsABC, suggesting that this region has diverged in mycoplasmas belonging to subspecies of M. capricolum.
dc.publisher Society for General Microbiology
dc.relation.isbasedon 10.1099/mic.0.25742-0
dc.title Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol trasnport locus (gtsABC)
dc.type Journal Article
dc.parent Microbiology-uk
dc.journal.volume 149
dc.journal.number en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 195 en_US
dc.identifier.endpage 204 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 107126
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)

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