Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues.

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dc.contributor.author Rice, SA
dc.contributor.author Koh, KS
dc.contributor.author Queck, SY
dc.contributor.author Labbate, M
dc.contributor.author Lam, KW
dc.contributor.author Kjelleberg, S
dc.date.accessioned 2010-05-28T09:45:08Z
dc.date.issued 2005-05
dc.identifier.citation Journal of bacteriology, 2005, 187 (10), pp. 3477 - 3485
dc.identifier.issn 0021-9193
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8733
dc.description.abstract We describe here a role for quorum sensing in the detachment, or sloughing, of Serratia marcescens filamentous biofilms, and we show that nutrient conditions affect the biofilm morphotype. Under reduced carbon or nitrogen conditions, S. marcescens formed a classical biofilm consisting of microcolonies. The filamentous biofilm could be converted to a microcolony-type biofilm by switching the medium after establishment of the biofilm. Similarly, when initially grown as a microcolony biofilm, S. marcescens could be converted back to a filamentous biofilm by increasing the nutrient composition. Under high-nutrient conditions, an N-acyl homoserine lactone quorum-sensing mutant formed biofilms that were indistinguishable from the wild-type biofilms. Similarly, other quorum-sensing-dependent behaviors, such as swarming motility, could be rendered quorum sensing independent by manipulating the growth medium. Quorum sensing was also found to be involved in the sloughing of the filamentous biofilm. The biofilm formed by the bacterium consistently sloughed from the substratum after approximately 75 to 80 h of development. The quorum-sensing mutant, when supplemented with exogenous signal, formed a wild-type filamentous biofilm and sloughed at the same time as the wild type, and this was independent of surfactant production. When we removed the signal from the quorum-sensing mutant prior to the time of sloughing, the biofilm did not undergo significant detachment. Together, the data suggest that biofilm formation by S. marcescens is a dynamic process that is controlled by both nutrient cues and the quorum-sensing system.
dc.format Print
dc.language eng
dc.relation.isbasedon 10.1128/jb.187.10.3477-3485.2005
dc.title Biofilm formation and sloughing in Serratia marcescens are controlled by quorum sensing and nutrient cues.
dc.type Journal Article
dc.parent Journal of bacteriology
dc.journal.volume 10
dc.journal.volume 187
dc.journal.number 10 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 3477 en_US
dc.identifier.endpage 3485 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 060503 Microbial Genetics
dc.personcode 106011
dc.percentage 100 en_US
dc.classification.name Microbial Genetics en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.location.activity United States
dc.description.keywords Biofilms
dc.description.keywords Carbon
dc.description.keywords Nitrogen
dc.description.keywords Culture Media
dc.description.keywords Signal Transduction
dc.description.keywords Serratia marcescens
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history Closed (ID: 3)


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