Definition of the attI1 site of class 1 integrons

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Show simple item record Partridge, SR Recchia, G Scaramuzzi, C Collis, CM Stokes, H Hall, RM 2010-05-28T09:45:09Z 2000-01
dc.identifier.citation Microbiology-uk, 2000, 146 (1), pp. 2855 - 2864
dc.identifier.issn 1350-0872
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Integron-encoded integrases recognize two distinct types of recombination site: attI sites, found in integrons, and members of the 59-base element (59-be) family, found in the integron-associated gene cassettes. The class 1 integron integrase, IntI1, catalyses recombination between attI1 and a 59-be, two 59-be, or two attI1 sites, but events involving two attI1 sites are less efficient than the reactions in which a 59-be participates. The full attI1 site is required for high-efficiency recombination with a 59-be site. It is 65 bp in length and includes a simple site, consisting of a pair of inversely oriented IntI1-binding domains, together with two further directly oriented IntI1-binding sites designated strong and weak. However, a smaller region that contains only the simple site is sufficient to support a lower level of recombination with a complete attI1 partner and the features that determine the orientation of attI1 reside within this region. An unusual reaction between the attI1 site and a 59-be appears to be responsible for the loss of the central region of a 59-be to create a potential fusion of two adjacent gene cassettes.
dc.format Scott McWhirter
dc.publisher Society for General Microbiology
dc.title Definition of the attI1 site of class 1 integrons
dc.type Journal Article
dc.parent Microbiology-uk
dc.journal.volume 1
dc.journal.volume 146
dc.journal.number en_US
dc.journal.number 1 en_US
dc.publocation United Kingdom en_US
dc.identifier.startpage 2855 en_US
dc.identifier.endpage 2864 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 105741
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords attI, integron, integrase, site-speci?c recombination, gene cassettes en_US
dc.description.keywords attI, integron, integrase, site-speci®c recombination, gene cassettes
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)

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