Early Targeting of Min Proteins to the Cell Poles in Germinated Spores of Bacillus subtilis: Evidence for Division Apparatus-Independent Recruitment of Min Proteins to the Division Site

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dc.contributor.author Harry, L
dc.contributor.author Lewis, PJ
dc.date.accessioned 2010-05-28T09:45:09Z
dc.date.issued 2003-01
dc.identifier.citation Molecular Microbiology, 2003, 47 (1), pp. 37 - 48
dc.identifier.issn 0950-382X
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8736
dc.description.abstract The earliest event in bacterial cell division is the assembly of a tubulin-like protein, FtsZ, at mid-cell to form a ring. In rod-shaped bacteria, the Min system plays an important role in division site placement by inhibiting FtsZ ring formation specifically at the polar regions of the cell. The Min system comprises MinD and MinC, which form an inhibitor complex and, in Bacillus subtilis, DivIVA, which ensures that division is inhibited only in the polar regions. All three proteins localize to the division site at mid-cell and to cell poles. Their recruitment to the division site is dependent on localization of both `early' and `late' division proteins. We have examined the temporal and spatial localization of DivIVA relative to that of FtsZ during the first and second cell division after germination and outgrowth of B. subtilis spores. We show that, although the FtsZ ring assembles at mid-cell about halfway through the cell cycle, DivIVA assembles at this site immediately before cell division and persists there during Z-ring constriction and completion of division. We also show that both DivIVA and MinD localize to the cell poles immediately upon spore germination, well before a Z ring forms at mid-cell. Furthermore, these proteins were found to be present in mature, dormant spores. These results suggest that targeting of Min proteins to division sites does not depend directly on the assembly of the division apparatus, as suggested previously, and that potential polar division sites are blocked at the earliest possible stage in the cell cycle in germinated spores as a mechanism to ensure that equal-sized daughter cells are produced upon cell division
dc.publisher Australian Society for Microbiology
dc.relation.isbasedon 10.1046/j.1365-2958.2003.03253.x
dc.title Early Targeting of Min Proteins to the Cell Poles in Germinated Spores of Bacillus subtilis: Evidence for Division Apparatus-Independent Recruitment of Min Proteins to the Division Site
dc.type Journal Article
dc.parent Molecular Microbiology
dc.journal.volume 1
dc.journal.volume 47
dc.journal.number 1 en_US
dc.publocation Australia en_US
dc.identifier.startpage 37 en_US
dc.identifier.endpage 48 en_US
dc.cauo.name SCI.Medical and Molecular Biosciences en_US
dc.conference Verified OK en_US
dc.for 0601 Biochemistry and Cell Biology
dc.for 0605 Microbiology
dc.personcode 995003
dc.percentage 50 en_US
dc.classification.name Biochemistry and Cell Biology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity en_US
dc.description.keywords en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)
utslib.collection.history Uncategorised (ID: 363)

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