The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR

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Show simple item record McKee, BA Djordjevic, SP Goodman, RD Hornitzky, MA 2010-05-28T09:45:13Z 2003-01
dc.identifier.citation Apidologie, 2003, 34 pp. 19 - 27
dc.identifier.issn 0044-8435
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract A hemi-nested polymerase chain reaction (PCR) was further developed for the detection of Melissococcus pluton in adult bees and honey bee products. A chloroform:isoamyl alcohol DNA extraction method was used to provide template from 154 samples of adult bee tissues, honey, pollen, whole larvae and comb cells. All 36 honey bee samples tested from a diseased colony were shown to contain M. pluton and sub-clinical infections were detected in adult bee tissues, larvae and honey (49/98; 50.0%) collected from all 9 healthy colonies from areas where EFB was endemic. All 20 adult bee tissue samples from a healthy colony from Western Australia where EFB has never been reported were negative. Of 80 bulk honey samples from six Australian states, 55 of 80 (68.8%) samples were shown to contain M. pluton whereas culture techniques detected M. pluton in 22 of 80 (27.5%) of these samples. M. pluton was detected in honey from all Australian states except Western Australia.
dc.publisher E D P Sciences
dc.relation.isbasedon 10.1051/apido:2002047
dc.title The detection of Melissococcus pluton in honey bees (Apis mellifera) and their products using a hemi-nested PCR
dc.type Journal Article
dc.parent Apidologie
dc.journal.volume 34
dc.journal.number en_US
dc.publocation France en_US
dc.identifier.startpage 19 en_US
dc.identifier.endpage 27 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0608 Zoology
dc.personcode 107126
dc.percentage 100 en_US Zoology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Open Access 2015-04-15 12:23:47.074767+10
pubs.consider-herdc false
utslib.collection.history General (ID: 2)

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