Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid

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Show simple item record Betteridge, T Yang, J Pittard, AJ Praszkier, J 2010-05-28T09:45:15Z 2004-01
dc.identifier.citation Journal Of Bacteriology, 2004, 186 (12), pp. 3785 - 3793
dc.identifier.issn 0021-9193
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract The replication initiator protein RepA of the IncB plasmid pMU720 was shown to induce localized unwinding of its cognate origin of replication in vitro. DnaA, the initiator protein of Escherichia coli, was unable to induce localized unwinding of this origin of replication on its own but enhanced the opening generated by RepA. The opened region lies immediately downstream of the last of the three binding sites for RepA (RepA boxes) and covers one turn of DNA helix. A 6-mer sequence, 5'-TCTTAA-3', which lies within the opened region, was essential for the localized unwinding of the origin in vitro and origin activity in vivo. In addition, efficient unwinding of the origin of replication of pMU720 in vitro required the native positioning of the binding sites for the initiator proteins. Interestingly, binding of RepA to RepA box 1, which is essential for origin activity, was not required for the localized opening of the origin in vitro.
dc.publisher Amer Soc Microbiology
dc.relation.isbasedon 10.1128/JB.186.12.3785-3793.2004
dc.title Role of RepA and DnaA proteins in the opening of the origin of DNA replication of an IncB plasmid
dc.type Journal Article
dc.parent Journal Of Bacteriology
dc.journal.volume 12
dc.journal.volume 186
dc.journal.number 12 en_US
dc.publocation Washington DC, USA en_US
dc.identifier.startpage 3785 en_US
dc.identifier.endpage 3793 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 060503 Microbial Genetics
dc.for 060501 Bacteriology
dc.personcode 106539
dc.percentage 50 en_US Microbial Genetics en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords NA en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)

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