The gene cassette metagenome is a basic resource for bacterial genome evolution

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Show simple item record Holmes, AJ Gillings, MR Nield, BS Mabbutt, BC Nevalainen, KMH Stokes, HW 2010-05-28T09:45:23Z 2003-05-01
dc.identifier.citation Environmental Microbiology, 2003, 5 (5), pp. 383 - 394
dc.identifier.issn 1462-2912
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Lateral gene transfer has been proposed as a fundamental process underlying bacterial diversity. Transposons, plasmids and phage are widespread and have been shown to significantly contribute to lateral gene transfer. However, the processes by which disparate genes are assembled and integrated into the host regulatory network to yield new phenotypes are poorly known. Recent discoveries about the integron/gene cassette system indicate it has the potential to play a role in this process. Gene cassettes are small mobile elements typically consisting of a promoterless orf and a recombination site. Integrons are capable of acquisition and re-arrangement of gene cassettes and of the expression of their associated genes. The potential of the integron/gene cassette system is thus largely determined by the diversity contained within the cassette pool and the rate at which integrons sample this pool. We show here using a polymerase chain reaction (PCR) approach by which the environmental gene cassette (EGC) metagenome can be directly sampled that this metagenome contains both protein-coding and non-protein coding genes. Environmental gene cassette-associated recombination sites showed greater diversity than previously seen in integron arrays. Class 1 integrons were shown to be capable of accessing this gene pool through tests of recombinational activity with a representative range of EGCs. We propose that gene cassettes represent a vast, prepackaged genetic resource that could be thought of as a metagenomic template for bacterial evolution.
dc.language eng
dc.relation.isbasedon 10.1046/j.1462-2920.2003.00429.x
dc.title The gene cassette metagenome is a basic resource for bacterial genome evolution
dc.type Journal Article
dc.parent Environmental Microbiology
dc.journal.volume 5
dc.journal.volume 5
dc.journal.volume 5
dc.journal.number 5 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 383 en_US
dc.identifier.endpage 394 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 104673
dc.personcode 105741
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity ISI:000182476100006 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history School of Medical and Molecular Sciences (ID: 341)
utslib.collection.history Closed (ID: 3)
utslib.collection.history Closed (ID: 3)

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