Twitching motility is essential for virulence in Dichelobacter nodosus

DSpace/Manakin Repository

Search OPUS


Advanced Search

Browse

My Account

Show simple item record

dc.contributor.author Han, X
dc.contributor.author Kennan, RM
dc.contributor.author Davies, JK
dc.contributor.author Reddacliff, LA
dc.contributor.author Dhungyel, OP
dc.contributor.author Whittington, RJ
dc.contributor.author Turnbull, L
dc.contributor.author Whitchurch, CB
dc.contributor.author Rood, JI
dc.date.accessioned 2010-05-28T09:45:24Z
dc.date.issued 2008-05
dc.identifier.citation Journal of Bacteriology, 2008, 190 (9), pp. 3323 - 3335
dc.identifier.issn 0021-9193
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8776
dc.description.abstract Type IV fimbriae are essential virulence factors of Dichelobacter nodosus, the principal causative agent of ovine foot rot. The fimA fimbrial subunit gene is required for virulence, but fimA mutants exhibit several phenotypic changes and it is not certain if the effects on virulence result from the loss of type IV fimbria-mediated twitching motility, cell adherence, or reduced protease secretion. We showed that mutation of either the pilT or pilU gene eliminated the ability to carry out twitching motility. However, the pilT mutants displayed decreased adhesion to epithelial cells and reduced protease secretion, whereas the pilU mutants had wild-type levels of extracellular protease secretion and adherence. These data provided evidence that PilT is required for the type IV fimbria-dependent protease secretion pathway in D. nodosus. It was postulated that sufficient fimbrial retraction must occur in the pilU mutants to allow protease secretion, but not twitching motility, to take place. Although no cell movement was detected in a pilU mutant of D. nodosus, aberrant motion was detected in an equivalent mutant of Pseudomonas aeruginosa. These observations explain how in D. nodosus protease secretion can occur in a pilU mutant but not in a pilT mutant. In addition, virulence studies with sheep showed that both the pilT and pilU mutants were avirulent, providing evidence that mutation of the type IV fimbrial system affects virulence by eliminating twitching motility, not by altering cell adherence or protease secretion. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
dc.language eng
dc.relation.isbasedon 10.1128/JB.01807-07
dc.title Twitching motility is essential for virulence in Dichelobacter nodosus
dc.type Journal Article
dc.description.version Published
dc.parent Journal of Bacteriology
dc.journal.volume 9
dc.journal.volume 190
dc.journal.number 9 en_US
dc.publocation Washington, USA en_US
dc.identifier.startpage 3323 en_US
dc.identifier.endpage 3335 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 103745
dc.personcode 103744
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000255202100026 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Uncategorised (ID: 363)
utslib.collection.history Closed (ID: 3)


Files in this item

This item appears in the following Collection(s)

Show simple item record