Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms

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dc.contributor.author Barken, KB
dc.contributor.author Pamp, SJ
dc.contributor.author Yang, L
dc.contributor.author Gjermansen, M
dc.contributor.author Bertrand, JJ
dc.contributor.author Klausen, M
dc.contributor.author Givskov, M
dc.contributor.author Whitchurch, CB
dc.contributor.author Engel, JN
dc.contributor.author Tolker-Nielsen, T
dc.date.accessioned 2010-05-28T09:45:36Z
dc.date.issued 2008-09
dc.identifier.citation Environmental Microbiology, 2008, 10 (9), pp. 2331 - 2343
dc.identifier.issn 1462-2912
dc.identifier.other C1UNSUBMIT en_US
dc.identifier.uri http://hdl.handle.net/10453/8814
dc.description.abstract When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can develop mushroom-shaped multicellular structures consisting of distinct subpopulations in the cap and stalk portions. We have previously presented evidence that formation of the cap portion of the mushroom-shaped structures in P. aeruginosa biofilms occurs via bacterial migration and depends on type IV pili (Mol Microbiol 50: 61-68). In the present study we examine additional factors involved in the formation of this multicellular substructure. While pilA mutants, lacking type IV pili, are deficient in mushroom cap formation, pilH and chpA mutants, which are inactivated in the type IV pili-linked chemosensory system, showed only minor defects in cap formation. On the contrary, fliM mutants, which are non-flagellated, and cheY mutants, which are inactivated in the flagellum-linked chemotaxis system, were largely deficient in cap formation. Experiments involving DNase treatment of developing biofilms provided evidence that extracellular DNA plays a role in cap formation. Moreover, mutants that are deficient in quorum sensing-controlled DNA release formed microcolonies upon which wild-type bacteria could not form caps. These results constitute evidence that type IV pili, flagellum-mediated motility and quorum sensing-controlled DNA release are involved in the formation of mature multicellular structures in P. aeruginosa biofilms. © 2008 The Authors.
dc.language eng
dc.relation.isbasedon 10.1111/j.1462-2920.2008.01658.x
dc.title Roles of type IV pili, flagellum-mediated motility and extracellular DNA in the formation of mature multicellular structures in Pseudomonas aeruginosa biofilms
dc.type Journal Article
dc.parent Environmental Microbiology
dc.journal.volume 9
dc.journal.volume 10
dc.journal.number 9 en_US
dc.publocation Oxford, UK en_US
dc.identifier.startpage 2331 en_US
dc.identifier.endpage 2343 en_US
dc.cauo.name SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 103745
dc.percentage 100 en_US
dc.classification.name Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US
dc.date.activity en_US
dc.location.activity ISI:000258203500013 en_US
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Strength - i3
utslib.copyright.status Closed Access
utslib.copyright.date 2015-04-15 12:17:09.805752+10
pubs.consider-herdc false
utslib.collection.history Closed (ID: 3)
utslib.collection.history Uncategorised (ID: 363)


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