The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO 1000 have a common origin

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Show simple item record Stokes, H Tomaras, C Parsons, Y Hall, RM 2010-05-28T09:45:49Z 1993-01
dc.identifier.citation Plasmid, 1993, 30 pp. 39 - 50
dc.identifier.issn 0147-619X
dc.identifier.other C1UNSUBMIT en_US
dc.description.abstract Integrons are genetic elements which are capable of acquiring genes by site-specific recombination. The most common integron structure consists of two conserved segments flanking a variable region where many different antibiotic resistance genes have been found. The integrons In6 and In7, present in the plasmids pSa and pDGO100, respectively, are unusual in that they include a duplication of the sulI gene which is located within the integron 3?-conserved segment. To further investigate the structure of these integrons, the DNA sequence of the segment located between the two sulI genes was determined. In In7 this segment is 2822 bases long and includes a trimethoprim resistance gene, dhfrX, at one end. The corresponding region in In6 is 4.5 kb and is nearly identical to the In7 segment over the first 2105 bases. In the region unique to In6, a cat gene, conferring chloramphenicol resistance, has replaced the dhfrX gene of In7. This location thus represents a second variable region where different antibiotic resistance genes are found, but the way in which genes become associated with this second variable region is not known. The overall similarity of the structures of In6 and In7 suggests that the additional DNA segments found in these integrons have a common origin, and a possible mechanism for the origin of integrons with partial 3?-conserved segment duplications is presented.
dc.format No Code
dc.publisher Academic Press
dc.relation.isbasedon 10.1006/plas.1993.1032
dc.subject NA, Microbiology
dc.subject NA; Microbiology
dc.title The partial 3'-conserved segment duplications in the integrons In6 from pSa and In7 from pDGO 1000 have a common origin
dc.type Journal Article
dc.parent Plasmid
dc.journal.volume 30
dc.journal.number en_US
dc.publocation United States en_US
dc.identifier.startpage 39 en_US
dc.identifier.endpage 50 en_US SCI.Institute for Biotechnology of Infectious Diseases en_US
dc.conference Verified OK en_US
dc.for 0605 Microbiology
dc.personcode 105741 en_US
dc.personcode 0000050369 en_US
dc.personcode 0000050370 en_US
dc.personcode 0000023445 en_US
dc.percentage 100 en_US Microbiology en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity en_US
dc.description.keywords NA en_US
dc.description.keywords NA
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science

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