Challenge of New Zealand Greenshell (TM) mussel Perna canaliculus larvae using two Vibrio pathogens: a hatchery study

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Show simple item record Kesarcodi-Watson, A Kaspar, H Lategan, MJ Gibson, L 2010-05-28T09:45:53Z 2009-01
dc.identifier.citation Disease of Aquatic Organisms, 2009, 86 (1), pp. 15 - 20
dc.identifier.issn 0177-5103
dc.identifier.other C1 en_US
dc.description.abstract Bacterial diseases remain a large problem in aquaculture hatcheries. The successful design and implementation of protective measures in the hatchery depends on an understanding of the dynamics of the infection process. Developing an in situ experimental protocol for pathogen challenge is therefore of paramount importance. Here, we demonstrated the minimum effective pathogenic dose (MEPD) of Vibrio splendidus (10(5) CFU ml(-1)) and a Vibrio coralliilyticus/neptunius-like isolate, Vibrio sp. DO1 (10(6) CFU ml(-1)), for New Zealand Greenshell (TM) mussel (GSM, Perna canaliculus) larvae during hatchery production. In a flow-through water hatchery system, larvae given 1 to 2 h of static water exposure to these pathogen doses showed respective average cumulative mortalities of 58 and 69 % on the fourth day following pathogen exposure. After the 1 to 2 h static exposure, larvae were returned to flow-through water. Larvae exposed to a dosage one order of magnitude greater than the MEPD had higher mortalities of 73 and 96 % for V. splendidus and Vibrio sp. DO1 respectively. These 4 levels of mortality were significantly greater than those of the non-exposed control larvae which respectively averaged 23 and 35 % in experiments involving V splendidus and Vibrio sp. DO1. Experiments were repeated 4 times to confirm reproducibility. After pathogen exposure, pathogens were detected in the larvae and tank water of treatments with dosages of >= 10(5) CFU ml-1 (V. splendidus) and 10(6) CFU ml(-1) (Vibrio sp. DO1), but not in treatments with lower pathogen dosages. The challenge protocols are reproducible and provide an opportunity to assess measures for the protection of GSM larvae against infection in the hatchery environment.
dc.publisher Inter-Research
dc.title Challenge of New Zealand Greenshell (TM) mussel Perna canaliculus larvae using two Vibrio pathogens: a hatchery study
dc.type Journal Article
dc.parent Disease of Aquatic Organisms
dc.journal.volume 1
dc.journal.volume 86
dc.journal.number 1 en_US
dc.publocation Oldendorf Luhe en_US
dc.identifier.startpage 15 en_US
dc.identifier.endpage 20 en_US SCI.Faculty of Science en_US
dc.conference Verified OK en_US
dc.for 070404 Fish Pests and Diseases
dc.personcode 004569
dc.personcode 102957
dc.percentage 100 en_US Fish Pests and Diseases en_US
dc.classification.type FOR-08 en_US
dc.edition en_US
dc.custom en_US en_US
dc.location.activity ISI:000270802000003 en_US
dc.description.keywords Vibriosis; Challenge protocol; Greenshell (TM) mussel; Larviculture en_US
dc.description.keywords Vibriosis
dc.description.keywords Challenge protocol
dc.description.keywords Greenshell (TM) mussel
dc.description.keywords Larviculture
pubs.embargo.period Not known
pubs.organisational-group /University of Technology Sydney
pubs.organisational-group /University of Technology Sydney/Faculty of Science
pubs.organisational-group /University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
utslib.copyright.status Closed Access 2015-04-15 12:17:09.805752+10
utslib.collection.history Closed (ID: 3)

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